Sheep anti Rabbit L-Lactic dehydrogenase LDH-5 (M4) isozyme, conjugated with Biotin, Primary Antibodies

• Catalog number
  NE094-Bio
• Price
  Please ask
• Size
  1ml

• Host Source
  Sheep
• Clone Name
  Polyclonal
• Product Category
  Enzymes, Veterinary
• Species Reactivity
  Rabbit
• Application
  ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
• Immunogen
  L-Lactic dehydrogenase, LDH-5(M4) isoenzyme, isolated and purified from rabbit muscle. FreundÍs complete adjuvant is used in the first step of the immunization procedure.
• Purification Method
  NA
• Shipping Conditions
  NA
• Storage conditions
  Sheep anti Rabbit L-Lactic dehydrogenase LDH-5 (M4) isozyme, conjugated with Biotin, Primary Antibodies philised conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4Á C--at least 10 years. Reconstituted at or below -20Á C--3-5 years. Reconstituted at +4Á C--7 days.
• Datasheet link
  NA
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• About
  Rabbits are used for polyclonal antibody production by Exalpha. Rabbit antibodies are very stable and can be stored for several days at room temperature. Exalpha adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

• Gene target
  L-Lactic   dehydrogenase   LDH-5   isozyme   conjugated   with   Primary   Antibodies  
• Gene symbol
  MIR1302-5, PIRC23, PIRC22, PIRC20, PIRC21, HTR6, IGHV2OR16-5, IGKV3OR2-5, SNORD116-5, TGFB1I1
• Short name
  anti Rabbit L-Lactic dehydrogenase LDH-5 (M4) isozyme, conjugated with , Primary Antibodies
• Technique
  Rabbit, anti, antibody to, antibody Conjugates
• Host
  Sheep, Rabbits
• Isotype
  NA
• Label
  Biotin
• Species
  Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
• Alternative name
  ovine antibody to production species: rabbit L-Lactic dehydrogenase LDH-5 (M4) isozyme, coupled including biotinilated, captor Antibodies
• Alternative technique
  rabbit-anti, antibodies

Gene info

• Identity
  HGNC:35297
• Gene
  MIR1302-5
• Long gene name
  microRNA 1302-5
• Synonyms gene
  • MIRN1302-5
• Synonyms
  • hsa-mir-1302-5
• Locus
  20q13.13
• Discovery year
  2008-11-03
• Entrez gene record
  100302146
• RefSeq identity
  • NR_031634
• Classification
  • MicroRNA MIR1302 family

Gene info

• Identity
  HGNC:37527
• Gene
  PIRC23
• Long gene name
  piwi-interacting RNA cluster 23
• Locus
  5
• Discovery year
  2009-11-05
• Entrez gene record
  100313786
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37526
• Gene
  PIRC22
• Long gene name
  piwi-interacting RNA cluster 22
• Locus
  5
• Discovery year
  2009-11-05
• Entrez gene record
  100313850
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37524
• Gene
  PIRC20
• Long gene name
  piwi-interacting RNA cluster 20
• Locus
  5
• Discovery year
  2009-11-05
• Entrez gene record
  100313785
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37525
• Gene
  PIRC21
• Long gene name
  piwi-interacting RNA cluster 21
• Locus
  5
• Discovery year
  2009-11-05
• Entrez gene record
  100313916
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:5301
• Gene
  HTR6
• Long gene name
  5-hydroxytryptamine receptor 6
• Synonyms gene name
  • 5-hydroxytryptamine (serotonin) receptor 6
  • 5-hydroxytryptamine (serotonin) receptor 6, G protein-coupled
• Synonyms
  • 5-HT6
  • 5-HT6R
• GenBank acession
  • L41147
• Locus
  1p36.13
• Discovery year
  1997-12-11
• Entrez gene record
  3362
• Pubmed identfication
  • 8522988
• RefSeq identity
  • NM_000871
• Classification
  • 5-hydroxytryptamine receptors, G protein-coupled
• VEGA ID
  OTTHUMG00000002713

Gene info

• Identity
  HGNC:5579
• Gene
  IGHV2OR16-5
• Long gene name
  immunoglobulin heavy variable 2/OR16-5 (non-functional)
• Synonyms gene name
  • immunoglobulin heavy variable 2/OR16-5
• Synonyms
  • IGHV2/OR16-5
• GenBank acession
  • L25544
• Locus
  16p11.2
• Discovery year
  2000-04-17
• Entrez gene record
  100129631
• Classification
  • Immunoglobulin heavy (IGH) orphons
• VEGA ID
  OTTHUMG00000152881

Gene info

• Identity
  HGNC:5832
• Gene
  IGKV3OR2-5
• Long gene name
  immunoglobulin kappa variable 3/OR2-5 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 3/OR2-5
  • immunoglobulin kappa variable 3/OR2-5 pseudogene
• Synonyms
  • IGKV3/OR2-5
• GenBank acession
  • X51882
• Locus
  2q11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28854
• Classification
  • Immunoglobulin kappa (IGK) orphons
• VEGA ID
  OTTHUMG00000184519

Gene info

• Identity
  HGNC:33071
• Gene
  SNORD116-5
• Long gene name
  small nucleolar RNA, C/D box 116-5
• Synonyms
  • HBII-85-5
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033417
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003320
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:11767
• Gene
  TGFB1I1
• Long gene name
  transforming growth factor beta 1 induced transcript 1
• Synonyms
  • Hic-5
  • TSC-5
  • ARA55
  • HIC-5
• Synonyms name
  • hydrogen peroxide-inducible clone-5
• GenBank acession
  • AB007836
• Locus
  16p11.2
• Discovery year
  1998-03-23
• Entrez gene record
  7041
• Pubmed identfication
  • 9422762
  • 10075738
• Classification
  • LIM domain containing
• VEGA ID
  OTTHUMG00000132467

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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