Helicobacter Rodentium PCR Positive Control
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples. Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts.
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Group
PCR, polymerase chain reaction, positif
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Short name
Helicobacter Rodentium PCR Positive Control
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Technique
Positive, Control, PCR, controls, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
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Species
Helicobacter, Helicobacter
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Alternative name
Helicobacter Rodentium PCR test kit Positive reference
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Alternative technique
controls, dna-amplification
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Tissue
control
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Disease
helicobacter, Helicobacter pillory antibodies and ELISA kits as diagnostic to detect Gram-negative helicobacter bacteria genus name Helicobacter.
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MeSH Data
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Name
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Concept
Scope note:
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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