UCH-L3 Deubiquitinase Inhibitor Screening Kit
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Catalog numberK471-100
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PricePlease ask
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Size100 assays
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Summary• Detection method- Fluorescence (RFU= 360/460 nm) • Application-Screening/characterizing/studying UCH-L3 inhibitors/activators
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Detection MethodFluorescence (RFU= 360/460 nm)
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ApplicationsScreening/characterizing/studying UCH-L3 inhibitors/activators
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Features Benefits• Simple and reliable test to screen UCH-L3 inhibitors/activators • High-throughput compatible • Includes Inhibitor Control
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Storage Conditions-20°C
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Shipping Conditionsgel pack
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Shelf life12 months
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BackgroundDiseases such as cancer and Alzehimer’s are often the result of dysregulation of proteostasis. Protein levels in the cell are regulated by both synthesis and degradation, with some proteins being marked for either localization or proteasomal degradation through the attachment of ubiquitin, a small, 76-amino acid protein. This attachment, termed ‘ubiquitination,’ or ‘ubiquitylation’ can be reversed by enzymes known as deubiquitinases (DUBs). The human proteome includes as many as four hundred DUBs, and thus these enzymes offer more specific targets for altering cellular equilibrium. UCH-L3, a DUB, is a cysteine protease that has been implicated in prostate cancer, although its regulation and downstream targets are largely unknown. It has been found that UCH-L3 is a novel regulator of epithelial-to-mesenchymal transition in cancer cell metastasis, making it an attractive drug target. Unfortunately, specific inhibitors for this and other DUBs are not readily available. Such compounds would also be a necessity for further illuminating and understanding downstream signaling. BioVision’s UCH-L3 Inhibitor Screening Kit utilizes the ability of active human UCH-L3 deubiquitinase to cleave a synthetic DUB substrate to release a fluorophore, which can be easily quantified (Ex/Em = 360/460 nm) using a fluorescence microplate reader. This inhibitor screening kit thus allows rapid and reliable determination of the inhibitory effects of various compounds on UCH-L3 deubiquitinase and could be used to screen for novel inhibitors. A nonspecific DUB inhibitor is included in this kit to allow the user to validate the experimental setup
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Additional descriptionTissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
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Gene target
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Gene symbolUCHL3, RPL3, LMO7-AS1, KLK9, UCHL1, MRPL38, CCR6, ADARB1
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Short nameUCH-L3 Deubiquitinase Inhibitor Screening Kit
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Alternative nameUCH-L3 Deubiquitinase suppressor detection reagent
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Alternative techniquekits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameubiquitin C-terminal hydrolase L3
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Synonyms gene name
- ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin C-terminal hydrolases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameribosomal protein L3
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-07-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNA protein coding host genes
- L ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameLMO7 antisense RNA 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2014-03-25
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Entrez gene record
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namekallikrein related peptidase 9
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Synonyms gene name
- kallikrein 9
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-03-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Kallikreins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameubiquitin C-terminal hydrolase L1
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Synonyms gene
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Synonyms gene name
- ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin C-terminal hydrolases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemitochondrial ribosomal protein L38
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-02-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitochondrial ribosomal proteins
- Large subunit mitochondrial ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine receptor 6
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Synonyms gene
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Synonyms gene name
- chemokine (C-C motif) receptor 6
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-04-21
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Entrez gene record
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Pubmed identfication
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Classification
- C-C motif chemokine receptors
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameadenosine deaminase RNA specific B1
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Synonyms gene name
- adenosine deaminase, RNA-specific, B1 (homolog of rat RED1)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-10-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Adenosine deaminases acting on RNA
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VEGA ID
