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Also known as
Actin, pan
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Other names
actin 5C, isoform C; Actin-5C; CG4027 gene product from transcript CG4027-RC; 5C actin; Act5C-PA; Act5C-PB; Act5C-PC; Act5C-PD; CG4027-PA; CG4027-PB; CG4027-PC; CG4027-PD; Dm-actin5C; F-actin; G-actin; actin 5 C; actin 5C; actin 5c; actin A1; anon-fast-evolving-2D2; cellular cytoskeletal beta-actin; Actin 5C; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
Act5C; Act5C; A; Ac5C; act; Act; act 5C; Act-5C; Act5; act5C; Act5c; ACT5C; actin; Actin; Actin/BAP47; actin5C; Actin5C; anon-EST:fe2D2; Bap47; BAP47; beta-actin; beta-actin/Bap47; CG4027; cyt5C; DmelCG4027; l(1)G0009; l(1)G0010; l(1)G0025; l(1)G0079; l(1)G0117; l(1)G0; N/A
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Gene name synonims
Act5C; Act5C; A; Ac5C; act; Act; act 5C; Act-5C; Act5; act5C; Act5c; ACT5C; actin; Actin; Actin/BAP47; actin5C; Actin5C; anon-EST:fe2D2; Bap47; BAP47; beta-actin; beta-actin/Bap47; CG4027; cyt5C; DmelCG4027; l(1)G0009; l(1)G0010; l(1)G0025; l(1)G0079; l(1)G0117; l(1)G0; N/A
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Other gene names
Act5C; Act5C; A; Ac5C; act; Act; act 5C; Act-5C; Act5; act5C; Act5c; ACT5C; actin; Actin; Actin/BAP47; actin5C; Actin5C; anon-EST:fe2D2; Bap47; BAP47; beta-actin; beta-actin/Bap47; CG4027; cyt5C; DmelCG4027; l(1)G0009; l(1)G0010; l(1)G0025; l(1)G0079; l(1)G0117; l(1)G0; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1,k
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Clone
3F12
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Host organism
Mouse (Mus musculus)
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Source organism
Chicken
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Species reactivity
Bovine, Canine, Chicken, Human (Homo sapiens), Mouse (Mus musculus), Porcine, Rabbit (Oryctolagus cuniculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Reacts with all six known vertebrate isoforms of actin (MW~42kD). It also reacts with two cytoplasmic actins (beta, gamma) which are highly homologous to one another, but which differ from the muscle actins by about 25 amino acid residues. This antibody is highly recommended for monitoring total protein load on Western blots. ; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Purified by Protein G affinity chromatography.
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Form Appearance
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See A0760-51
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze atStore the antibody at -20 degrees Celsius. or colder. Aliquots are stable for at least 12 months atStore the antibody at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested applications
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Description
Sodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. MBS Monoclonals supplies azide free antibodies on request. Recently MBS Monoclonals made this Actin, pan (BSA & ) Antibody available without azide. It will stick less and is more suitable for ELISA or in vivo studies. However you need to store the @VV@ frozen and ship on dry ice.
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French translation
anticorps
