-
Category
Primary Antibodies
-
Long description
CD1 molecules are 49kDa type 1 membrane glycoproteins that contain a single Ig-like domain, which associate with beta-2 microglobulin to form a heterodimer. The CD1 glycoproteins function as antigen-presenting molecules for exogenous and endogenous lipids, glycolipids and lipopeptides to specialised T cells. The CD1 molecules show some sequence similarities to major histocompatibility (MHC) class I and class II molecules, but are not encoded by the MHC genes and are not significantly polymorphic. In humans, the CD1 genes encode five isoforms of CD1, namely CD1a-e, with each type reported to show a different pattern of expression on cells and within different tissues. CD1a, b and c belong to group 1 of the CD1 family and are expressed by dendritic cells, activated monocytes and other antigen-presenting cells. CD1a is also expressed by some malignancies of T cell lineage and is commonly used as a marker of dendritic cell populations.
-
Antibody come from
CBT6 is a mouse monoclonal IgG1 antibody derived by the fusion of NS0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a T-lymphocyte derived antigen.
-
Other description
Each vial contains 500 µl 0.2 mg/ml purified monoclonal antibody in phosphated buffered saline (PBS) containing 50% glycerol + 0.2% bovine serum albumin (BSA) + 0.02% sodium azide.
-
Clone
CBT6
-
Antigen antibody binding interaction
Mouse anti CD1a Antibody
-
Antibody is raised in
Mouse
-
Antibody s reacts with
Human, Mouse
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
The CBT6 antibody is directed against human CD1a and has been reported to cross react with mouse thymocytes.
-
Research interest
CD Marker, Immunology
-
Application
ELISA, Flow cytometry, Immunocytochemistry, Immunohistochemistry (frozen)
-
Antibody s suited for
The CBT6 antibody is suitable for the detection of CD1a by ELISA and flow cytometry. The antibody is also suitable for the detection of CD1a by immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilutions for the different applications should be determined by titration.
-
Storage
Store at 4oC, or in small aliquots at -20oC.
-
Relevant references
1. Yakimchuk K, Roura-Mir C, Magalhaes KG, de Jong A, Kasmar AG, Granter SR, Budd R, Steere A, Pena-Cruz V, Kirschning C, Cheng TY, Moody DB (2011) Borrelia burgdorferi infection regulates CD1 expression in human cells and tissues via IL1-β. Eur J Immunol. 41:694-705. 2. Goan SR, Junghahn I, Wissler M, Becker M, Aumann J, Just U, Martiny-Baron G, Fichtner I, Henschler R (2000) Donor stromal cells from human blood engraft in NOD/SCID mice. Blood. 96:3971-8.
-
Protein number
UniProt: P06126 (Human)
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
