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Description
Plasmid maxiPREP Kit is used for isolating the plasmid DNA from the cultured bacterial cells.
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Storage
Store at room temperature.
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Shelflife
Use before expiry date given on the label
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Sample
Up to 200 ml bacterial cells
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Format
NA
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Operation time
NA
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Elution volume
NA
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Yield
Up to 850 μg of plasmid
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Contents
Buffer M1 Buffer M2 Buffer M3 Buffer W1 Buffer W2 *Add 100 ml of ethanol prior to initial use. Buffer E RNase A (50mg/ml) MX Column
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PHYSICAL AND CHEMICAL PROPERTIES
Buffer M1 Form: liquid Miscibility with water: immiscible pH：7.6 // Buffer M2 Form: liquid Miscibility with water: immiscible pH：13 // Buffer M3 Form: liquid Miscibility with water: immiscible pH：4.5 // Buffer W1 Form: liquid Miscibility with water: immiscible pH：7.0 // Buffer W2 Form: liquid Miscibility with water: immiscible pH：7.5 // BufferE Form: liquid Miscibility with water: immiscible pH：8.5 // RNase A Form: liquid Miscibility with water: immiscible pH：6.8
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Uses
For laboratory use
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Ordering
To order Plasmid maxiPREP Kit, please use the Cat. Nr.PDP03-0010 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Plasmid maxiPREP Kit
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Kit
Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
