DFF45 Antibody
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Catalog number3048-100
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PricePlease ask
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Size100 µg
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Antibody TargetDFF45
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Target Alternative NameDFFA, DFF-45, ICAD, DFF1
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Antibody TypePolyclonal
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Species ReactivitiesMouse, rat
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Immunogen SequenceSynthetic peptide mapping to the N-terminus of mouse DFF45
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AccessionAAH58213
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Gene Id13347
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AppearanceColorless liquid
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FormLiquid
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Concentration0.2 mg/ml
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Formulation100 µg (0.2 mg/ml) immunoaffinity purified rabbit DFF45 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot
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Application And UsagesWestern blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the 45 kDa and 28 kDa proteins, corresponding to the apparent molecular weight of DFF45/ICADL, and ICADS in immunoblots.
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DescriptionApoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as many other types of diseases. Recently, a human DNA fragmentation factor (DFF45) was identified. DFF is composed of two subunts: DFF40 and DFF45. Cleavage of DFF45, which is mediated by caspase-3, leads to DFF40’s activation as a nuclease. Activation of DFF40 can lead to DNA fragmentation, a hallmark of apoptosis.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Dot Blot VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolDFFA
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Short nameDFF45 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameDFF45 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameDNA fragmentation factor subunit alpha
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Synonyms gene name
- DNA fragmentation factor, 45 kD, alpha polypeptide
- DNA fragmentation factor, 45kDa, alpha polypeptide
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data