FITC-Linked Polyclonal Antibody to Activated Leukocyte Cell Adhesion Molecule (ALCAM)

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• Catalog number
  MBS2034719
• Price:

  536.6 USD

  Ask for price
• Size
  0,1 mg

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• Other size
  please contact us to order other different size
• Description
  cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the FITC-Linked Polyclonal Antibody Activated Leukocyte Adhesion Molecule (ALCAM). For cells, cell lines and tissues in culture till half confluency. Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits.
• Properties
  If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

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• Gene target
  Linked   Activated   Leukocyte   Adhesion   Molecule   ALCAM  
• Gene symbol
  ALCAM
• Short name
  FITC-Linked Polyclonal Antibody Activated Leukocyte Adhesion Molecule (ALCAM)
• Technique
  Polyclonal, Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Label
  FITC
• Alternative name
  fluorecein-Linked polyclonal (antibody to-) to Activated Leukocyte cellular Adhesion Molecule (activated leukocyte cellular adhesion molecule)
• Alternative technique
  polyclonals, antibodies, fluorescine
• Alternative to gene target
  activated leukocyte cell adhesion molecule, CD166 and MEMD, ALCAM and IDBG-48383 and ENSG00000170017 and 214, protein binding, Cell surfaces, Alcam and IDBG-165765 and ENSMUSG00000022636 and 11658, ALCAM and IDBG-631637 and ENSBTAG00000000088 and 281614
• Tissue
  cell

Gene info

• Identity
  HGNC:400
• Gene
  ALCAM
• Long gene name
  activated leukocyte cell adhesion molecule
• Synonyms gene name
  • activated leucocyte cell adhesion molecule
• Synonyms
  • CD166
  • MEMD
• GenBank acession
  • AK054632
• Locus
  3q13.11
• Discovery year
  1997-01-17
• Entrez gene record
  214
• Pubmed identfication
  • 7760007
• RefSeq identity
  • NM_001627
• Classification
  • Ig-like cell adhesion molecule family
  • C2-set domain containing
  • V-set domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000159192

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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