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Available ordering format
Liquid in PBS, pH 7.4, with 0.02% sodium azide
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Immunogen
KLH-conjugated N-terminal peptide of Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria
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Raised in
Mouse
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Clonality
Monoclonal
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Clone
Clone GF28R
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Purification
protein G purified
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Antibody can be stored at -20°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
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Verified applications
Western Blot (WB), ELISA (ELISA), Immunohistochemistry (IHC), Dot Blot
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Connected products
other tag antibodiesSecondary antibodies
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Recommended dilutions for use
1:1000 (WB)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Native and denaturated Cyan Fluorescent protein (CFP) and its variants: Enhanced Green Fluorescent Protein (EGFP), Yellow Fluorescent Protein (YEP)
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Possible reactivity
to be determined
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No reactivity
no confirmed exceptions from predicted reactivity are currently known
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Supplementary information
protein G purified
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References
to be added when available antibody available in October 2015.
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Scientific context
CFP (Cyan fluorescent protein) is a naturally fluorescent protein which emits blue light when excited by a 405 nm laser. This emission is optimally detected at 485 nm. It is used in laboratories as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications.
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Notes
exact working dilution needs to be determined by end user
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Test
Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
