Rabbit Anti-Human ATG4D Polyclonal: ATTO 680
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Catalog numberSPC-635D-A680
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Price:
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Size100 µg
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ImmunogenSynthetic peptide from the N-terminal of Human ATG4D (aa. 98-108)
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Antibody s targetHuman ATG4D
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Antibody s full descriptionRabbit Anti-Human ATG4D Polyclonal
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Primary research fieldsCardiovascular System, Heart, Cancer, Autophagy
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameAutophagin-4 Antibody, APG4D Antibody, ATG4D Antibody, Cysteine protease ATG4D Antibody, AUTL4 Antibody, Autophagy-related cysteine endopeptidase 4 Antibody, EC:3.4.22.- Antibody, AUT-like 4 cysteine endopeptidase Antibody, Autophagy-related protein 4 homolog D Antibody,
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Verified applicationsWB
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Raised inRabbit
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Antibody s reactivityHuman
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Antibody s recommended dilutions for useWB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~53 kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1000 dilution of SPC-635 was sufficient for detection of ATG4D in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm
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Tissue specificityMainly expressed in skeletal muscle and, to a lower extent, in testis.
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Scientific contextATG4D, Auotphagy related 4D, cysteine peptidases, belong to the autophagy-related protein 4 family of C54 endopeptidases. Members of this family play a role in the biogenesis of autophagosomes (1). Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (2).
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Bibliography1. "Entrez Gene: Autophagy related 4D, cysteine peptidase". 2. Li M., et al. (2011) J Biol Chem. 286: 7327-7338.
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Released date29/Aug/2016
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NCBI numberNP_001268433.1
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Gene number84971
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Protein numberQ86TL0
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure legendWestern blot analysis of Human HeLa cell lysates showing detection of 52.9 kDa ATG4D protein using Rabbit Anti-ATG4D Polyclonal Antibody (SPC-635). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody (SPC-635) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 52.9 kDa. Other Band(s): ~45 and 48 kDa. Rabbit Anti-ATG4D Antibody used in Western blot (WB) on HeLa cell lysates (SPC-635)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolATG4D
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Short nameRabbit Anti- ATG4D Polyclonal: ATTO 680
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelATTO 680
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman ATG4D polyclonal: ATTO 680
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene nameautophagy related 4D cysteine peptidase
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Synonyms gene
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Synonyms gene name
- AUT-like 4, cysteine endopeptidase (S. cerevisiae)
- APG4 autophagy 4 homolog D (S. cerevisiae)
- ATG4 autophagy related 4 homolog D (S. cerevisiae)
- autophagy related 4D, cysteine peptidase
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-04-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
- Autophagy related
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data