pCMV-N-FLAG-RELB Plasmid

  • Catalog number
    PVTB00501-2a
  • Price
    381.42 USD
  • Size
    2 ug
  • Properties
    An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
  • Conjugation
    Flag
  • Kit
    Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
  • Gene target
    pCMV-N   RELB  
  • Gene symbol
    RELB
  • Short name
    pCMV-N- -RELB Plasmid
  • Technique
    plasmid, plasmids in 1
  • Label
    Flag
  • Alternative name
    pCMV-N-FLAG-v-v-rel reticuloendotheliosis viral oncogene homolog (avian) reticuloendotheliosis viral oncogene homolog B Plasmid
  • Alternative technique
    plasmids
  • Alternative to gene target
    v-rel reticuloendotheliosis viral oncogene homolog B, I-REL and IREL and REL-B, RELB and IDBG-57021 and ENSG00000104856 and 5971, protein binding, nuclei, Relb and IDBG-151419 and ENSMUSG00000002983 and 19698, RELB and IDBG-639330 and ENSBTAG00000038428 and 522670
Gene info
  • Identity
  • Gene
  • Long gene name
    RELB proto-oncogene, NF-kB subunit
  • Synonyms gene name
    • v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1995-10-02
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • IPT domain containing
    • NF-kappa B complex subunits
  • VEGA ID
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