Sonic Hedgehog (SHH), murine recombinant
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Catalog number4020-1000
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Price:
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Size1 mg
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SynonymsSHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog
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Alternative_namesSHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog
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DescriptionHedgehog signaling pathway ligand involved in regulating vertebrate organogenesis
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥97%
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AssaySDS-PAGE
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Purity≥97%
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Biological activityMouse SHH is fully biologically active when compared to standard. Determined by it’s ability to induce alkaline phosphatase production by C3H10T1/2 fibroblasts, with an expected ED₅₀ in the range of 0.03 – 0.30 ug/ml.
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Results0.03 – 0.30 ug/ml.
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Molecular Weight19.8 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionLyophilized from a 10 mM sodium phosphate buffer, pH 7.5.
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Reconstitution InstructionsWhen reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile H₂O to a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions.
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Background InformationRecombinant Mouse Sonic Hedgehog is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Mouse Sonic Hedgehog is 99% homologous to the human gene. Cysteine at position 25 has been substituted with Ile. Mouse SHH is a non-glycosylated polypeptide consisting of 176 amino acids with a molecular weight of 19,800 Da.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolSHH
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Short nameSonic Hedgehog (SHH), murine recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative nameSonic Hedgehog (sonic hedgehog), murine Rec.
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Alternative techniquerec
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Alternative to gene targetsonic hedgehog, HHG1 and HLP3 and HPE3 and MCOPCB5 and SMMCI and TPT and TPTPS, SHH and IDBG-50464 and ENSG00000164690 and 6469, laminin-1 binding, nuclei, Shh and IDBG-144309 and ENSMUSG00000002633 and 20423, SHH and IDBG-638249 and ENSBTAG00000024552 and
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Gene info
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Identity
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Gene
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Long gene namesonic hedgehog signaling molecule
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Synonyms gene
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Synonyms gene name
- sonic hedgehog (Drosophila) homolog
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Synonyms
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Locus
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Discovery year1995-03-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Hedgehog signaling molecule family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data