Goat anti-Biotin - Affinity Pure, DyLight350 Conjugate
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Catalog number
MBS686141
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Price
Please ask
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Size
1 mg
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Products_type
Antibody
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Products_short_name
[Goat anti-Biotin]
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Products_name_syn
[Goat anti-Biotin - Affinity Pure]
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Reactivity
Biotin. Cross Reactivity: This antibody does not react with free BSA or free KLH
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Specificity
This antibody reacts with Biotin
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Purity
Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase biotin.
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Form
Lyophilized
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Concentration
1.0 mg/ml (E 1% at 280 nm = 13.0)
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Storage_stability
Store freeze-dried powder at 2-8 degree C. Shelf Life: Product is stable for up to 4 weeks at 2-8 degree C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20 degree C.
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Tested_application
Flow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM)
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Test
A high affinity purification column was use to purify Goat anti- - Pure, DyLight350 Conjugate by MyBioSource by chromatographic size exclusion.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure Goat anti- - Pure, DyLight350 Conjugate supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Latin name
Capra aegagrus hircus
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Gene target
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Short name
Goat anti- - Pure, DyLight350 Conjugate
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Technique
affinity, anti-, anti, Goat, Pure, antibody to, antibodies, goats, pure products
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Label
Biotin
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Species
Goats
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Alternative name
caprine Antibody tobiotinilated - protein Pure, DyLight350 Conjugate
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Alternative technique
antibodies, purified, caprine
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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