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Products_type
Secondary Antibody
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Reactivity
Goat.Cross Reactivity: Based on IEP, no reactivity is observed to:non-immunoglobulin goat serum immunoglobulinsIgG from human, mouse, rabbit or rat
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Test
A high affinity purification column was use to purify anti-Goat IgG (H L) - Pure, FITC Conjugate, min x w/ , mouse, rabbit IgG[IgG (H L)] by MyBioSource by chromatographic size exclusion. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure anti-Goat IgG (H L) - Pure, FITC Conjugate, min x w/ , mouse, rabbit IgG[IgG (H L)] supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.
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Latin name
Equus africanus asinus, Capra aegagrus hircus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Short name
anti-Goat IgG (H L) - Pure, FITC Conjugate, min x w/ , mouse, rabbit IgG[IgG (H L)]
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Technique
affinity, Rabbit, anti-, Mouse, anti, FITC, Goat, Pure, IgG, antibody to, antibodies, Fluorescein, goats, IgGs, mouses, pure products
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Host
Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Isotype
IgG, Heavy & Light chains, IgG
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Label
FITC
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Species
Rat, Donkeys, Goats, Humans, Mouses, Rats
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Alternative name
Donkey Antibody toGoat Immunoglobulin G (heavy + light chain) - protein Pure, fluorecein Conjugate, min x w/H. sapiens, mouse, production species: rabbit and rat Immunoglobulin G[Immunoglobulin G (heavy + light chain)]
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Alternative technique
rabbit-anti, murine, antibodies, purified, caprine, fluorescine, immunoglobulins
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