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Alternative name1
Anti-GOAT F(ab')2 ANTI HAMSTER IgG:FITC
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Alternative name2
Caprine F(ab')2 ANTI HAMSTER IgG:FITC
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Alternative name3
Caprinae F(ab')2 ANTI HAMSTER IgG:FITC
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Alternative name4
IgG
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Alternative name5
N/A
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Other names
N/A
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Gene name
IgG
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Gene name synonims
N/A
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Other gene names
N/A
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Category
Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
Polyclonal IgG
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Clone
Polyclonal antibody
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Host organism
Goat
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Species reactivity
Hamster
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Specificity and cross reactivity
IgG This item recognises hamster IgG. The reagent has been adsorbed against both mouse and rat immunoglobulins to minimise cross-reactivity.
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Purification method
N/A
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Form Appearance
FITC (F(ab')2 fragment of purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
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Concentration
IgG concentration 0.8 mg/ml
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Storage and shipping
Store at +Store productone at +4 degrees Celsius. or productone should be stored at -20 degrees Celsius. if preferred. productone should be stored undiluted. Storage in frost free freezers is not recommended. productone is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should productone contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of dispatch.
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Tested for
Flow Cytometry (FACS)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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Group
f(ab')2s
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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French translation
anticorps
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Short name
GOAT F(ab')2 ANTI IgG:FITC Antibody
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Technique
Antibody, F(ab')2, anti, FITC, Goat, IgG, antibody to, antibodies against human proteins, antibodies for, F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. Fluorescein, goats, IgGs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, F(ab')2 Fragment Affinity-Purified Secondary Antibodies, IgG
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Label
FITC
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Species
Hamster, Goats, Hamsters
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Alternative name
caprine F(Antibody')2 antibody to HAMSTER Immunoglobulin G:fluorecein (antibody to-)
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Alternative technique
antibodies, secondairy, caprine, fluorescine, immunoglobulins
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