MOUSE ANTI HUMAN CD53:FITC

• Catalog number
  MBS211079
• Price
  Please ask
• Size
  100 Tests

• Products_type
  Antibody
• Products_short_name
  [CD53]
• Other_names
  [leukocyte surface antigen CD53; Leukocyte surface antigen CD53; leukocyte surface antigen CD53; CD53 antigen; CD53 glycoprotein; CD53 tetraspan antigen; antigen MOX44 identified by monoclonal antibody MRC-OX44; cell surface antigen; cell surface glycoprotein CD53; tetraspanin-25; transmembrane glycoprotein; tspan-25; CD53 molecule; Cell surface glycoprotein CD53; Tetraspanin-25; Tspan-25; CD_antigen: CD53]
• Products_gene_name
  [CD53]
• Other_gene_names
  [CD53; CD53; MOX44; TSPAN25; MOX44; TSPAN25; Tspan-25]
• Clonality
  Monoclonal
• Clone
  [MEM-53]
• Specificity
  This item reacts with a 32kDa antigen, a single chain glycoprotein on most leucocytes. There is no cross-reactivity with erythrocytes or platelets. The antibody recognises a broad span of leucocytes of both normal and neoplastic origin.
• Form
  FITC Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration
  IgG concentration 0.1 mg/ml
• Storage_stability
  Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
• Tested_application
  Flow cytometry (FC/FACS)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  CD53  
• Gene symbol
  CD53
• Short name
  MOUSE ANTI CD53:FITC
• Technique
  anti human, Mouse, anti, FITC, antibody to, anti human antibodies, Fluorescein, mouses
• Host
  Mouse
• Isotype
  IgG1
• Label
  FITC
• Species
  Human, Humans, Mouses
• Alternative name
  MOUSE antibody to H. sapiens CD53 molecule:fluorecein
• Alternative technique
  murine, antibodies, fluorescine
• Alternative to gene target
  CD53 molecule, MOX44 and TSPAN25, CD53 and IDBG-101004 and ENSG00000143119 and 963, protein binding, Cell surfaces, Cd53 and IDBG-184902 and ENSMUSG00000040747 and 12508, CD53 and IDBG-635180 and ENSBTAG00000006466 and 505040

Gene info

• Identity
  HGNC:1686
• Gene
  CD53
• Long gene name
  CD53 molecule
• Synonyms gene
  • MOX44
• Synonyms gene name
  • CD53 antigen
• Synonyms
  • TSPAN25
• GenBank acession
  • BC040693
• Locus
  1p13.3
• Discovery year
  1991-07-16
• Entrez gene record
  963
• Pubmed identfication
  • 8319976
• RefSeq identity
  • NM_000560
• Classification
  • Tetraspanins
  • CD molecules
• VEGA ID
  OTTHUMG00000048020

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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