Mothers Against Decapentaplegic Homolog 5 (Smad5) Polyclonal Antibody (Human), FITC

• Catalog number
  PAC122Hu01-200ul-FITC
• Price
  Please ask
• Size
  200ul

• Description
  A Rabbit polyclonal antibody against Human Mothers Against Decapentaplegic Homolog 5 (Smad5). This antibody is labeled with FITC.
• Specifications
  Host: Rabbit; Species Reactivity: Human; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
• Additional_information
  Sequence of the immunogen: Smad5 (Gly210~Val464); Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Storage_and_shipping
  Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
• Notes
  Research Use Only.
• Properties
  If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Anti-FITC Antibody
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  Mothers   Against   Decapentaplegic   Homolog   Smad5  
• Gene symbol
  SMAD5
• Short name
  Mothers Against Decapentaplegic Homolog 5 (Smad5) Polyclonal Antibody ( ), FITC
• Technique
  Polyclonal, Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Label
  FITC
• Species
  Human, Humans
• Alternative name
  Mothers Against Decapentaplegic Homolog 5 (SMAD family member 5) polyclonal (antibody to-) (H. sapiens), fluorecein
• Alternative technique
  polyclonals, antibodies, fluorescine
• Alternative to gene target
  SMAD family member 5, DWFC and JV5-1 and MADH5, SMAD5 and IDBG-45903 and ENSG00000113658 and 4090, transforming growth factor beta receptor, nuclei, Smad5 and IDBG-157537 and ENSMUSG00000021540 and 17129, BT.69069 and IDBG-645857 and ENSBTAG00000021772 and 768050

Gene info

• Identity
  HGNC:6771
• Gene
  SMAD5
• Long gene name
  SMAD family member 5
• Synonyms gene
  • MADH5
• Synonyms gene name
  • MAD, mothers against decapentaplegic homolog 5 (Drosophila)
  • SMAD, mothers against DPP homolog 5 (Drosophila)
• Synonyms
  • Dwfc
  • JV5-1
• GenBank acession
  • U59913
• Locus
  5q31.1
• Discovery year
  1997-07-01
• Entrez gene record
  4090
• Pubmed identfication
  • 8673135
• RefSeq identity
  • NM_005903
• Classification
  • SMAD family
• VEGA ID
  OTTHUMG00000163212

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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