HIF-1-Alpha isoform 1 + 2 Antibody Western Blot Positive Control

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Description

The HIF-1-Alpha isoform 1 + 2 Antibody Blot Positive Control is a α- or alpha protein sometimes glycoprotein present in blood. Isotype or positive controls by peptides, antibodies and deactivated samples. Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts.
Properties

If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Test

Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
Group

positif
French translation

anticorps

Gene target

HIF-1-Alpha isoform
Gene symbol

HIF1A, SETD2, ARNT, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1
Short name

HIF-1-Alpha isoform 1 + 2 Antibody Blot Positive Control
Technique

Western Blot, Antibody, Positive, Control, Blot, antibodies against human proteins, antibodies for, western Blots, controls
Alternative name

HIF-1-a isoform 1 + 2 (antibody to-) Western Blot Positive reference
Alternative technique

controls, antibodies, western
Tissue

control

Identity

HGNC:4910
Gene

HIF1A
Long gene name

hypoxia inducible factor 1 subunit alpha
Synonyms gene name
- hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Synonyms
- MOP1
- HIF-1alpha
- PASD8
- HIF1
- bHLHe78
Synonyms name
- PAS domain-containing protein 8”, “Class E basic helix-loop-helix protein 78”, “Member of PAS protein 1
GenBank acession
- U22431
Locus

14q23.2
Discovery year

1995-03-27
Entrez gene record

3091
Pubmed identfication
- 8786149
- 9079689
RefSeq identity
- NM_001530
Classification
- Basic helix-loop-helix proteins
VEGA ID

OTTHUMG00000140344

Identity

HGNC:5670
Gene

IGHVII-1-1
Long gene name

immunoglobulin heavy variable (II)-1-1 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
Synonyms
- IGHV(II)-1-1
GenBank acession
- AB019441
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28377
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000152325

Identity

HGNC:5760
Gene

IGKV1OR2-1
Long gene name

immunoglobulin kappa variable 1/OR2-1 (pseudogene)
Synonyms gene
- IGKVPZ1
- IGKV1OR-1
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
Synonyms
- IGKV1/OR-1
- IGKV1/OR2-1
GenBank acession
- Z12367
Locus

2p11.1
Discovery year

2000-04-18
Entrez gene record

3531
Pubmed identfication
- 2853098
Classification
- Immunoglobulin kappa (IGK) orphons
VEGA ID

OTTHUMG00000155081

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data