SIRT1 Antibody

• Catalog number
  6137-100
• Price
  Please ask
• Size
  100 μg

• Antibody Target
  SIRT1
• Target Alternative Name
  SIRT1, SIR2L1
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  C terminal peptide of human SIRT1
• Accession
  Q96EB6
• Gene Id
  23411
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
• Purification
  Affinity purified
• Application
  Western blot, Immunohistochemistry
• Positive Control
  Western Blot: Various cell lysates
• Application And Usages
  Western blot: 1:500 – 1:2000 dilution, IHC: 1:50 - 1:200 dilution. However, the optimal conditions should be determined individually.
• Description
  The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group. This SIRT family of genes is highly conserved from prokaryotes to eukaryotes and is involved in diverse processes including transcriptional regulation, cell cycle progression, DNA damage repair and aging. SIRT1 has been shown to be induced by caloric restriction (CR) in several tissues and SIRT1 mediated deacetylase activity attenuates B-amyloid content in the brain during CR. SIRT1 also regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  SIRT1  
• Gene symbol
  SIRT1, SIRT1-AS
• Short name
  SIRT1 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  sirtuin 1 (antibody to-)
• Alternative technique
  antibodies
• Alternative to gene target
  sirtuin 1, SIR2L1, SIRT1 and IDBG-75847 and ENSG00000096717 and 23411, keratin filament binding, nuclei, Sirt1 and IDBG-159441 and ENSMUSG00000020063 and 93759, SIRT1 and IDBG-629119 and ENSBTAG00000014023 and 613629

Gene info

• Identity
  HGNC:14929
• Gene
  SIRT1
• Long gene name
  sirtuin 1
• Synonyms gene name
  • sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1
  • sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
• Synonyms
  • SIR2L1
• GenBank acession
  • AF083106
• Locus
  10q21.3
• Discovery year
  2001-03-20
• Entrez gene record
  23411
• Pubmed identfication
  • 10381378
• RefSeq identity
  • NM_012238
• Classification
  • eNoSC complex
  • Sirtuins
• VEGA ID
  OTTHUMG00000018340

Gene info

• Identity
  HGNC:51912
• Gene
  SIRT1-AS
• Long gene name
  SIRT1 antisense RNA
• Locus
  10q21.3
• Discovery year
  2015-09-23
• Entrez gene record
  106633813
• Pubmed identfication
  • 26324025
• Classification
  • Antisense RNAs

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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