pIMAGO-biotin HRP on Western Blot
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Catalog number
194-800-10
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Price
Please ask
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Size
1 vial
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Ordering information
The catalog number at Gentaur for this research reagent is 194-800-10. It is a frequent ordered item.
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Delivery
The pIMAGO-biotin HRP on Western Blot can be delivered to your laboratory the next week after your order if you order before Friday 15:00.
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Users reference
Contact us for laboratory references of researchers that recently used this product in Europe or the United States.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
Biotinylated, HRP Conjugated
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Test
Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
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Gene target
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Short name
pIMAGO- on Blot
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Technique
Western Blot, Blot, western Blots
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Label
Biotin
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Alternative name
pIMAGO-biotinilated horseradish peroxidase on Western Blot
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Alternative technique
western
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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