pIMAGO-biotin HRP on Western Blot
-
Catalog number
194-800-10
-
Price
Please ask
-
Size
1 kit or item
-
-
How to order
The reference at Genprice for this lab product is 194-800-10. It is a frequent ordered reagent.
-
When will I be delivered
The pIMAGO-biotin HRP on Western Blot will be delivered to your laboratory the next week after your order if you order it before Friday 15:30.
-
Contact with other users
pIMAGO-biotin HRP on Western Blot is frequently used in research laboratories. We can supply you with references if needed.
-
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
-
Conjugation
Biotinylated, HRP Conjugated
-
Test
Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
-
Gene target
-
Short name
pIMAGO- on Blot
-
Technique
Western Blot, Blot, western Blots
-
Label
Biotin
-
Alternative name
pIMAGO-biotinilated horseradish peroxidase on Western Blot
-
Alternative technique
western
-
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products