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Also known as
CD81/TAPA-1
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Other names
CD81 antigen; CD81 antigen; CD81 antigen; tspan-28; tetraspanin-28; OTTHUMP00000011528; OTTHUMP00000011529; OTTHUMP00000229004; OTTHUMP00000229005; OTTHUMP00000229006; OTTHUMP00000229008; OTTHUMP00000229358; OTTHUMP00000229392; 26 kDa cell surface protein TAPA-1; CD81 antigen (target of antiproliferative antibody 1); CD81 molecule; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28
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Category
Secondary Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
CD81; CD81; S5.7; CVID6; TAPA1; TSPAN28; TAPA1; TSPAN28
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Gene name synonims
CD81; CD81; S5.7; CVID6; TAPA1; TSPAN28; TAPA1; TSPAN28
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Other gene names
CD81; CD81; S5.7; CVID6; TAPA1; TSPAN28; TAPA1; TSPAN28
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Clonality
Monoclonal
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Immunoglobulin isotype
Armenian Hamster IgG, group 3, kappa
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Clone
2F7
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Host organism
Armenian Hamster
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Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Mouse CD81 (Mr 25 kDa), a member of the transmembrane 4 integral membrane protein family CD81/TAPA-1 is an integral membrane protein expressed on a variety of cell types, and has a high degree of sequence homology between human and mouse.1 CD81 is expressed on thymic stromal cells where it plays an important role in the transition of gamma delta + T cells to more mature T cells with alpha beta T cell receptors.2 Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor, which is composed of a TCR beta chain and glycoprotein pT alpha.2 The monoclonal antibody 2F7 can block thymocyte interaction with CD81 in vitro.2; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
R - phycoerythrin (R - PE) Conjugate
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Concentration
N/A
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Storage and shipping
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below Store the antibody at -20 degrees Celsius.. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product the antibody should be stored at -20 degrees Celsius. immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
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Tested applications
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
CD81-AS1, ETV3, ERF, CD81
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Short name
Anti-Mouse CD81-PE
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Technique
anti-, Mouse, anti, antibody to, antibodies, mouses
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Host
Hamster
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Label
PE
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Species
Mouse, Hamsters, Mouses
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Alternative name
Anti-Hamster Anti-Mouse CD81-PE
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Alternative technique
murine, antibodies
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Alternative to gene target
CD81 molecule, CVID6 and S5.7 and TAPA1 and TSPAN28, CD81 and IDBG-22455 and ENSG00000110651 and 975, MHC class II protein complex binding, Cell surfaces, Cd81 and IDBG-212677 and ENSMUSG00000037706 and 12520, CD81 and IDBG-645214 and ENSBTAG00000047495 and 511435
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