M-CSF Recombinant Protein
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Catalog number
40-479
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Price
Please ask
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Size
0.002 mg, 0.01 mg
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About recombinant protein
M-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. The human is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant rat M-CSF is a 18.1 kDa homodimeric protein containing two 155 amino acid polypeptide subunits.
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Other name of the recombinant
Csfm, Macrophage colony-stimulating factor 1, CSF-1
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Recombinant in
E. coli
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SEQUENCE
MEVSEHCSHM IGNGHLQILQ QLIDSQMETA CLIEYKFVDQ EQLDDPVCYL KKAFVLVQVI IEETMRFKDN TPNANATERL QELSMKLNSC FIKDYKEQNE ACVQTYKESP LRLLEKIKNF FNETKNFLEK DWNIFSKNCN DSLAKCSSRD VVTKP
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Activity
The ED50 was determined by the dose - dependent stimulation of the proliferation of murine M - NFS - 60 cells is < 5.0 ng/mL, corresponding to a specific activity of > 1 x 105 units/mg.
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Keep at
The lyophilized M-CSF recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted M-CSF is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing M-CSF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI
50054384
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Accession nr
NP_076471.3
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Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
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About
This product is for research use only.
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Protein s origin
Rat
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Vial
Lyophilized
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NCBI ID
78965
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Gene name
colony stimulating factor 1 (macrophage)
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Source
Rattus norvegicus
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Uniprot
Q8JZQ0
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
Csf1
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Short name
M-CSF Recombinant Protein
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
M-CSF Rec. Protein
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Alternative technique
rec
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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