HTT Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

DNA lentivector for transduction information

Lentiveral packaging plasmid DNA for non-viral plasmid transfection and direct use in plasmid expression. This DNA can alos be used for packaging into Lentiviral particles for high efficiency transduction and stably integrated expressions. GENTAUR suggests to use our ABM packaging mix LV003 of second generation virusses or the LV053, our 3rd Generation Packaging mixture. pLenti lentiviral plasmids DNAs are stored in 10milliMolar Tris/HCI with 1mM EDTA at a pH of 8 at -25 C. Vectors with selection markers like kanamycin, puromycin or cumate are available.
Lentiviral technical files
Lentivirus references
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Gene target

HTT Lentiviral Vector CMV pLenti-GIII-CMV-RFP-2A-Puro
Gene symbol

HTT-AS, SLC6A4, TRIM27, HTT, IRX5, PLA2G3, IGHD2OR15-2A, EIF2A, CXCL2, RNVU1-2A
Short name

HTT Lentiviral Vector ( ) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
Technique

Vectors
Label

puro
Species

Human, Humans
Alternative name

huntingtin Lentiviral integrating Desoxyribonucleic acid sequence (H. sapiens) (cytomegalovirus) (pLenti-GIII-cytomegalovirus-Red Fluorescent-2A-Puro)
Alternative to gene target

huntingtin, HD and IT15, HTT and IDBG-7852 and ENSG00000197386 and 3064, diazepam binding, nuclei, Htt and IDBG-156033 and ENSMUSG00000029104 and 15194, HTT and IDBG-643549 and ENSBTAG00000001506 and 615059
Virus

cmv

Identity

HGNC:37118
Gene

HTT-AS
Long gene name

HTT antisense RNA
Synonyms gene
- HTT-AS1
Synonyms gene name
- HTT antisense RNA 1 (non-protein coding)
- HTT antisense RNA 1
- HTT antisense RNA (head to head)
Synonyms
- HTTAS
Locus

4p16.3
Discovery year

2011-06-30
Entrez gene record

100750326
Pubmed identfication
- 21672921
- 23756188
RefSeq identity
- NR_045414
Classification
- Antisense RNAs
VEGA ID

OTTHUMG00000159915

Identity

HGNC:11050
Gene

SLC6A4
Long gene name

solute carrier family 6 member 4
Synonyms gene
- HTT
- OCD1
Synonyms gene name
- solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
- 5-hydroxytryptamine (serotonin) transporter
- obsessive-compulsive disorder 1
- solute carrier family 6 (neurotransmitter transporter), member 4
Synonyms
- 5-HTT
- SERT1
Synonyms name
- serotonin transporter 1
GenBank acession
- L05568
Locus

17q11.2
Discovery year

1994-03-16
Entrez gene record

6532
Pubmed identfication
- 7681602
- 19806148
RefSeq identity
- NM_001045
Classification
- Solute carriers
VEGA ID

OTTHUMG00000132751

Name

Transduction, Genetic
Concept

Scope note: The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
Tree numbers
- E05.393.350.800
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data