Target of this antibody:
Alternative names of antibody target:
TRK , TRKA , Trk-A , MTC , CIPA , NTRK1 , MTC , TRK1 , p140-TrkA , DKFZp781I14186
TrkA, the high affinity Nerve Growth Factor (NGF) receptor, autophosphorylates on tyrosine to activate multiple effectors. Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at these sites reflects Trk kinase activity.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic phospho-peptide corresponding to resides surrounding Tyr490 of human TrkA
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-Phospho-TrkA polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunofluorescence
Western blotting (1 µg/ml), immunoprecipitation (4-8 µg/ml), and immunocytochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 140 kDa TrkA only when phosphorylated at Tyr490.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.