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Alternative_name
Ac-VDVAD-AFC, Caspase-2 Substrate, Fluorogenic, VDVAD-AFC, caspase2 substrate, caspase 2 substrate
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Description
Ready-to-use fluorometric substrate for caspases that recognize the amino acid sequence VDVAD. Caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate VDVAD-AFC at Ex/Em = 400/505 nm using a fluorometer or a fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat.# 1067-100), 2X Reaction Buffer (Cat.# 1068-20), DTT (Cat.# 1201-1), and other reagents used for caspase activity assays are also available separately.
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Structure Available
No
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Peptide sequence
Ac-Val-Asp-Val-Ala-Asp-AFC
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Salt Form
No
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Molecular Formula
C₃₃H₄₁F₃N₆O₁₂
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Molecular Weight
770.7
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Cell Permeable
No
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Purity
>98% by HPLC
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Handling
Protect from light and moisture
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Tag Line
A caspase-2 substrate
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
12 months
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MDL Number
MFCD04037014
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PubChem CID
44135100
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SMILES
CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC(C(C)C)C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C
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InChi
InChI=1S/C33H41F3N6O12/c1-13(2)26(38-16(6)43)32(53)41-21(12-24(46)47)30(51)42-27(14(3)4)31(52)37-15(5)28(49)40-20(11-23(44)45)29(50)39-17-7-8-18-19(33(34,35)36)10-25(48)54-22(18)9-17/h7-10,13-15,20-21,26-27H, 11-12H2,1-6H3,(H,37,52)(H,38,43)(H,39,50)(H,40,49)(H,41,53)(H,42,51)(H,44,45)(H,46,47)
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InChi Key
BKQMJQIAIFEZJR-UHFFFAOYSA-N
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MSDS Available
Yes
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Additional description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.