pCR-BluntII-TOPO-LGR5
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Catalog numberPVT14395
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Price530.64 USD
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Size2 ug
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GroupPCR, polymerase chain reaction
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AboutTAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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PropertiesThermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Gene symbolLGR5
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Short namepCR-BluntII-TOPO-LGR5
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TechniquePCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
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Alternative namePCR test kit-BluntII-TOPO-LGR5
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Alternative techniquedna-amplification
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Gene info
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Identity
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Gene
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Long gene nameleucine rich repeat containing G protein-coupled receptor 5
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Synonyms gene
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Synonyms gene name
- G protein-coupled receptor 49
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-10-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- G protein-coupled receptors, Class A orphans
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VEGA ID
MeSH Data
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Name
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ConceptScope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
- E05.393.620.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data