Superoxide Dismutase (SOD), human recombinant
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Catalog number4802-100
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Price:
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Size100 ug
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SynonymsSuperoxide dismutase [Cu-Zn], EC 1.15.1.1, SOD1, SOD, ALS, ALS1, IPOA
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Alternative_namesSuperoxide dismutase [Cu-Zn], EC 1.15.1.1, SOD1, SOD, ALS, ALS1, IPOA
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DescriptionEnzyme that catalyze the dismutation of superoxide
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥98%
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AssaySDS-PAGE
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Purity≥98%
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Biological activityBioVision recombinant Superoxide Dismutase is fully biologically active when compared to standard. Activity tests were carried using SOD activity assay kit (K335-100). The activity assay kit showed that the calculated activity was ~40,000 U/mg. This was calculated from the amount (µg) required to inhibit the Xanthine Oxidase activity by 50%
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Unit DefinitionOne unit is defined as the amount of enzyme that will cause a 50% reduction of the rate of WST-1 formazan formation
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Molecular Weight16.8 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionLyophilized powder
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Reconstitution InstructionsReconstitute in H₂O to a concentration >100 ug/ml. The solution can then be diluted into other aqueous buffers
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Background InformationSuperoxide Dismutase (SOD) is an oxidoreductase that catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide. Recombinant Human Cu/Zn Superoxide Dismutase produced in E.Coli is a homodimer, non-glycosylated polypeptide chain containing 2 x 154 amino acids and having a total molecular mass of 31,608 Dalton. The Cu/Zn SOD is purified by proprietary chromatographic techniques.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolSOD3
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Short nameSuperoxide Dismutase (SOD), recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameSuperoxide Dismutase (antioxidant enzyme), H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namesuperoxide dismutase 3
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Synonyms gene name
- superoxide dismutase 3, extracellular
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Synonyms
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Locus
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Discovery year1988-05-08
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Entrez gene record
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Classification
- Superoxide dismutases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data