Urokinase, human recombinant

• Catalog number
  7696-500
• Price
  Please ask
• Size
  500 µg

• Synonyms
  Two chain urokinase-type plasminogen activator (tcuPA), PLAU, ATF, UPA, URK, u-PA, BDPLT5，QPD
• Alternative_names
  Two chain urokinase-type plasminogen activator (tcuPA), PLAU, ATF, UPA, URK, u-PA, BDPLT5，QPD
• Description
  A potent marker of invasion and metastasis in a variety of human cancers
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  The enzyme is fully active as seen from its ability to cleave a fluorogenic substrate N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC) (Cat # K728-100).
• Biological activity
  >1500 mU/mg (1 U = Digestion of 1 µmole of Z-GGR-AMC substrate in 1 min at 37ºC.)
• Results
  >1500 mU/mg (1 U = Digestion of 1 µmole of Z-GGR-AMC substrate in 1 min at 37ºC.)
• Unit Definition
  1 U = Digestion of 1 µmole of Z-GGR-AMC substrate in 1 min at 37ºC
• Molecular Weight
  The active Urokinase is a two-chain glycoprotein and has a calculated MW of 49.3 kDa. DTT-reduced Protein migrates as two bands corresponding to the two chains with the molecular mass of 28.3 and 21 kDa.
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Lyophilized from proprietary buffer.
• Reconstitution Instructions
  Briefly spin down the vial and reconstitute in water to 0.5-1 mg/ml and store at –80°C.
• Background Information
  Urokinase or Urokinase-type plasminogen activator (uPA) is a serine protease (EC 3.4.21.73). It is secreted as a single-chain zymogen, pro-Urokinase, possessing little or no intrinsic enzymatic activity. The single chain zymogen is converted into the active two chain enzyme (tcuPA) by cleavage of the bond between Lys158 and Ile159. After activation, Urokinase specifically cleaves the proenzyme plasminogen to form the active enzyme plasmin. The active plasmin then catalyzes the breakdown of fibrin polymers of blood clots. Urokinase is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing. Additionally, it is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Urokinase  
• Gene symbol
  PLAUR, PLAU
• Short name
  Urokinase, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Urokinase, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:9053
• Gene
  PLAUR
• Long gene name
  plasminogen activator, urokinase receptor
• Synonyms
  • URKR
  • UPAR
  • CD87
• Synonyms name
  • urokinase-type plasminogen activator (uPA) receptor
  • urokinase plasminogen activator surface receptor
• Locus
  19q13
• Discovery year
  1991-06-05
• Entrez gene record
  5329
• RefSeq identity
  • NM_002659
• Classification
  • LY6/PLAUR domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000182778
• Locus Specific Databases
  • LRG_637

Gene info

• Identity
  HGNC:9052
• Gene
  PLAU
• Long gene name
  plasminogen activator, urokinase
• Synonyms
  • URK
  • UPA
• GenBank acession
  • BC013575
• Locus
  10q22.2
• Discovery year
  2001-06-22
• Entrez gene record
  5328
• Pubmed identfication
  • 2415429
• RefSeq identity
  • NM_002658
• VEGA ID
  OTTHUMG00000018494
• Locus Specific Databases
  • LRG_593

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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