Ga15/16 Antibody
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Catalog number3250R-30T
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PricePlease ask
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Size30 µg
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Antibody TargetGa 15/16
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Target Alternative NameG alpha binding protein
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceSynthetic peptide surrounding amino acid 356of human G 15
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AccessionAAC25612
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Gene Id2769
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation30 µg (0.5 mg/ml) affinity purified rabbit anti-Gα15 polyclonal antibody in PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot
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Application And UsagesWestern blotting (0.5-4 µg/ml) and in Immunohistochemistry (5-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects Gα15 in samples from human, mouse, rat, and rabbit origins. It also detects Gα16 from samples of human origin. Reactivity to other species has not been determined.
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DescriptionGα16 is the only heterotrimeric G protein with a restricted expression pattern in hematopoietic cells. Differentiation of promyelocytic cells leads to decreased expression of Gα16. The G protein is known to couple a large number of G protein-coupled receptors to PLC-β and can lead to production of the secondary messengers diacylglycerol and inositol phosphates. Targeted deletion of Gα15, a mouse orthologue of human Gα16, causes reduced C5α receptor signaling. Gα16 serves as a marker, in addition to CD34, for hematopoietic progenitor cells.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Short nameGa15/16 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameGa15/16 (antibody to-)
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data