pCMV-MTOR-Flag Plasmid
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Catalog numberPVTB00953-2a
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Price380.85 USD
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Size2 ug
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PropertiesAn anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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ConjugationFlag
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KitPlasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
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Gene target
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Gene symbolMTOR-AS1, MTOR
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Short namepCMV-MTOR- Plasmid
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Techniqueplasmid, plasmids in 1
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LabelFlag
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Alternative namepCMV-Mammalian target on rapamycin-Flag Plasmid
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Alternative techniqueplasmids
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Alternative to gene targetmechanistic target of rapamycin (serine/threonine kinase), FRAP and FRAP1 and FRAP2 and RAFT1 and RAPT1, MTOR and IDBG-89258 and ENSG00000198793 and 2475, transferase activity, nuclei, Mtor and IDBG-204403 and ENSMUSG00000028991 and 56717, MTOR and IDBG-632731 and ENSBTAG00000015325 and 100139219
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Gene info
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Identity
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Gene
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Long gene nameMTOR antisense RNA 1
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Synonyms gene name
- MTOR antisense RNA 1 (non-protein coding)
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Locus
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Discovery year2011-05-18
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemechanistic target of rapamycin kinase
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Synonyms gene
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Synonyms gene name
- FK506 binding protein 12-rapamycin associated protein 1
- mechanistic target of rapamycin (serine/threonine kinase)
- mechanistic target of rapamycin
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Synonyms
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Synonyms name
- FK506 binding protein 12-rapamycin associated protein 2
- rapamycin target protein
- FKBP12-rapamycin complex-associated protein 1
- FKBP-rapamycin associated protein
- rapamycin associated protein FRAP2
- dJ576K7.1 (FK506 binding protein 12-rapamycin associated protein 1)
- rapamycin and FKBP12 target 1
- mammalian target of rapamycin
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GenBank acession
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Locus
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Discovery year1995-07-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MTOR complex 1
- MTOR complex 2
- Armadillo like helical domain containing
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VEGA ID
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Locus Specific Databases