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Alternative_name
2,2'-(1,2-hydrazinediylidene)bis[3-ethyl-2,3-dihydro-6-benzothiazolesulfonic acid, diammonium salt
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Description
ABTS is considered a safe and sensitive substrate compatible for horseradish peroxidase (HRP) based ELISA assays. In the presence of hydrogen peroxide and HRP, ABTS is oxidized to a radical cation with an absorption maxima at 820 nm, 734 nm, 650 nm and 405 nm. The latter frequency demonstrates a significant molar extinction coefficient and is generally employed for most ABTS assays. Stopping the reaction with acid does not alter the 405 nm spectrum allowing kinetic and endpoint methods to be measured at the same wavelength.
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CAS Number
30931-67-0
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Structure Available
Yes
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Salt Form
No
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Molecular Formula
C₁₈H₂₄N₆O₆S₄
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Molecular Weight
548.68
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Purity
≥98% by HPLC
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Solubilities
~10 mg/ml, DMSO >40 mg/ml Water
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Handling
Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
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Tag Line
A peroxidase substrate
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Storage Condition
-20ºC
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Shipping Condition
Gel Pack
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Shelf Life
36 months
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MDL Number
MFCD00010404
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PubChem CID
9570474
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SMILES
[O-]S(C1=CC=C(N(CC)/C(S2)=N/N=C3SC(C=C(S([O-])(=O)=O)C=C4)=C4N\3CC)C2=C1)(=O)=O.[NH4+].[NH4+]
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InChi
InChI=1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
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InChi Key
OHDRQQURAXLVGJ-AXMZSLBLSA-N
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MSDS Available
1
