Glutaminase (GLS1) Inhibitor Screening Kit (Fluorometric)
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Catalog number
K479-100
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Price
Please ask
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Size
100 assays
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Description
Sensitive, reproducible assay for initial screening of potential Glutaminase Inhibitors
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Detection Method
Fluorometric (Ex/Em = 535/587 nm)
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Species Reactivity
Human
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Applications
Screening of potential inhibitors of Glutaminase
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Features Benefits
Sensitive, specific assay for screening GLS1 inhibitors, Includes hrGLS1 and CB-839 (inhibitor control)
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Storage Conditions
-20°C
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Shipping Conditions
Gel Pack
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Shelf life
12 months
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Background
Glutaminase (EC 3.5.1.2, GLS) is an enzyme that hydrolyzes glutamine producing glutamate and ammonia. It has tissue-specific roles in multiple organs, some of which include ammonia generation for urea synthesis in the liver; maintenance of acid-base homeostasis by ammonia production during renal acidosis in the kidney; and regulation of neurotransmitter glutamate in the brain. The isoform glutaminase 1 (GLS1) or phosphate-activated mitochondrial glutaminase is critical for glutamine utilization by cancer cells and the rate of glutaminolysis is known to increase in tumors, making it extremely important for tumor cell metabolism. GLS1 inhibitors have been considered as potential candidate drugs for cancer therapy. BioVision’s Glutaminase (GLS1) Inhibitor Screening Kit is a plate-based fluorometric kit for screening human GLS1 inhibitors. The hydrolysis of glutamine by glutaminase forms glutamate and ammonia. Glutamate, in the presence of a developer and enzyme mix, converts a non-fluorescent probe to a fluorescent product via an enzymatic reaction. A well-known Glutaminase inhibitor - CB-839 is provided as an inhibitor control.
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Additional description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
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Gene target
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Gene symbol
GLS
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Short name
Glutaminase (GLS1) Inhibitor Screening Kit (Fluorometric)
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Alternative name
Glutaminase (GLS1) suppressor detection reagent (fluorescence)
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Alternative technique
kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
glutaminase
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Synonyms
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GenBank acession
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Locus
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Discovery year
1989-02-07
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Entrez gene record
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Pubmed identfication
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Classification
- Ankyrin repeat domain containing
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VEGA ID
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