CSTA, human recombinant

• Catalog number
  9236-10
• Price
  Please ask
• Size
  10 µg

• Synonyms
  Cystatin-A, STF1, STFA
• Alternative_names
  Cystatin-A, STF1, STFA
• Description
  A thiol protease inhibitors that form complexes with papain and the cathepsins B, H and L
• Recombinant
  Yes
• Source
  E.coli
• Purity by SDS PAGE
  >95%
• Assay
  SDS-PAGE
• Purity
  >95%
• Biological activity
  IC50 value is < 1.0nM
• Molecular Weight
  This protein is fused with a 6× His tag at N-terminus and has a calculated MW of 13.1 kDa.
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  Liquid
• Reconstitution Instructions
  In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol
• Background Information
  CSTA, also known as Stefin A, is thiol protease inhibitors that form complexes with papain and the cathepsins B, H and L. The protein is one of the precursor proteins of the cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. CSTA is thought to be a tumor suppressor, since an inverse correlation seems to exist between the level of CSTA and tumor progression, in several malignancies
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MIPGGLSEAK PATPEIQEIV DKVKPQLEEK TNETYGKLEA VQYKTQVVAG TNYYIKVRAG DNKYMHLKVF KSLPGQNEDL VLTGYQVDKN KDDELTGF
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CSTA  
• Gene symbol
  CSTA
• Short name
  CSTA, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  CSTA, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:2481
• Gene
  CSTA
• Long gene name
  cystatin A
• Synonyms gene
  • STF1
  • STFA
• Synonyms gene name
  • cystatin A (stefin A)
• Synonyms name
  • stefin A
• Locus
  3q21.1
• Discovery year
  1990-02-06
• Entrez gene record
  1475
• Pubmed identfication
  • 1674139
• RefSeq identity
  • NM_005213
• Classification
  • Cystatins, type 1
• VEGA ID
  OTTHUMG00000159488

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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