HSP27 Antibody

• Catalog number
  bs-0730R
• Price
  Please ask
• Size
  0.1ml

• Long name
  HSP27 Primary Polyclonal Antibody
• Also known as
  HSP27 PAb
• Category
  Primary Antibodies
• Conjugation
  Unconjugated
• Target Antigen
  HSP27
• Specificity
  This is a highly specific antibody against HSP27.
• Modification s
  None
• Modification site s
  Unmodified antibody
• Clonality
  Polyclonal antibody
• Clone number
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen range
  100-150/205
• Subcellular location
  Cytoplasm, Nucleus
• Source
  This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human HSP27
• Gene ID number
  3315
• Swiss Prot
  P04792
• Tested Applications
  WB, IHC-P, IF(IHC-P)
• Recommended dilutions
  WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
• Cross reactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the target antigen
  Heat Shock Protein 27 (HSP27) is a 27 kDa member of a family of proteins whose expression and function are stimulated by heat shock and other stress stimuli. A major function of these proteins is to serve as chaperones that bind to and stabilize the active conformation of other proteins. HSP27, along with other members of the small HSP group, possesses a C-terminal Alpha-crystalline homology domain. HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Cytoplasmic HSP27 exists in multiple complexes. One complex consists of HSP27, Akt (PKB), MAPKAP-kinase 2, and p38 MAPK. The presence of HSP27 in this complex is required for Akt activation by stress stimuli. Another complex consists of HSP27 and the IKK complex. HSP27 is also an actin capping protein that binds to the barbed (growing) ends of actin filaments, thereby inhibiting filament extension. Phosphorylation of HSP27 on serine 82 by MAPKAP-kinase 2 leads to HSP27 dissociation from the Akt/MAPKAP-kinase 2/p38 complex and from actin filaments, and stimulates HSP27 binding to the IKK complex.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
• Synonym names
  CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.7667; HEL-S-12; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27
• Properties
  If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  HSP27  
• Gene symbol
  HSPB1
• Short name
  Anti-HSP27
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Host
  Rabbit (Oryctolagus cuniculus)
• Isotype
  Immunoglobulin G (IgG)
• Alternative name
  HSP27 Polyclonal Antibody
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:5246
• Gene
  HSPB1
• Long gene name
  heat shock protein family B (small) member 1
• Synonyms gene name
  • heat shock 27kD protein 1
  • heat shock 27kDa protein 1
• Synonyms
  • HSP27
  • HSP28
  • Hs.76067
  • Hsp25
  • CMT2F
• GenBank acession
  • X54079
• Locus
  7q11.23
• Discovery year
  1991-07-09
• Entrez gene record
  3315
• Pubmed identfication
  • 2243808
  • 9344682
• Classification
  • Small heat shock proteins
• VEGA ID
  OTTHUMG00000023228
• Locus Specific Databases
  • Inherited Peripheral Neuropathies Mutation Database
  • LRG_248

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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