Human Recombinant Cpn10 Protein
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Catalog numberSPR-310C
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Price:
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Size2x100 µg
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Stock availabilityIn Stock
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Scientific contextChaperonin 10, otherwise known as Cpn10, (groES in E.coli) make up a family of small heart shock proteins with an approximate molecular mass of 10kDa (HSP10s). Cpn10 acts as a co-chaperone and interacts with the HSP60 family to promote proper folding of polypeptides. Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly process (1). Cpn10 has been located in human platelets, but is also present in human maternal serum (2, 3). It has been reported that human Cpn10 is identical with early pregnancy factor, which is involved in control over cell growth and development. This identification suggest that Cpn10 may act like a hormone in stressful situations such as pregnancy (4).
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Protein targetCpn10
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Protein reactivityHuman
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Certificate of analysisThis product has been certified >90% pure using SDS-PAGE analysis.
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Protein descriptionHuman Recombinant Cpn10 Protein
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Other name10kDa Chaperonin Protein, Chaperonin 10 Protein, Cpn 10 Protein, EPF Protein, GROES Protein, Heat Shock 10kD protein1 Protein, HSP10 Protein, HSPE1 Protein
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Primary research areaCancer, Heat Shock
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CategoryProtein
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Brand namenone
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OriginRecombinant
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NCBI numberNM_002157.2
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Gene number3336
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Protein numberP61604
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Verified applicationsWB, SDS-PAGE
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Relevant bio activityCpn10 Protein
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Protein expression modelE. coli
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Protein charastericsSee included datasheet.
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Peptide sequenceMAGQAFRKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQATVVAVGSGSKGKGGEIQPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDGDILGKYVD
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Protein purificationMulti-Step Purified
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Purity pourcentage>90% High purity
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Recommended buffer for storage20mM Tris, pH7.5, 0.3M NaCl, 10% glycerol, 1 mM DTT
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Protein concentrationLot/batch specific. See included datasheet.
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Protein specificity~10 kDa
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Protein tagNo tag
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Supplementary useful informationPlease see included datasheet or contact us
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Protein cell localizationMitochondrion Matrix
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Bibliography1. Velez-Granell C.S., et al. (1994) J of Cell Science. 107(3):539-549. 2. Morton H., Hegh V., and Clunie G.J.A. (1974) Nature (London) 249: 459-460. 3. Cavanagh A.C., and Morton H. (1994) Eur. J. Biochem. 222: 551-560. 4. Minto M., et al. (1998) Molecular Cell Research. 1403 (2): 151-157.
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Release date11-Sep-2009
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PubMed number23775284
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Tested applicationsFunctional Assay
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Tested species reactivityHuman
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Representative figure legendSDS-PAGE of 10kDa human Cpn10 protein (SPR-310). SDS-Page of human Cpn10 Protein (SPR-310)
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WarningsNon-hazardous materials
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Protein originCanada
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Total weight kg1.4
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Net weight g0.2
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolHSPE1
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Short nameRecombinant Cpn10 Protein
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. StressMark proteins advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameH. sapiens Rec. Cpn10 Protein
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameheat shock protein family E (Hsp10) member 1
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Synonyms gene name
- heat shock 10kD protein 1 (chaperonin 10)
- heat shock 10kDa protein 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1994-11-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chaperonins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data