References of collagen and chondrocyte research and assays
Kim W.U., Yoo W.H., Won Park, Kang Y.M., Kim S.I., Park J.H., Lee S.S., Joo Y.S, Min J. K., Hong Y.S., Lee S.H., Park S.H., Cho C.S., Kim H.Y. IgG antibodies to type II collagen reflect inflammatory activity in patients with rheumatoid arthritis. Journal of Rheumatology, 2000; 27(3):575581. Terato K., DeArmey D.A., Ye X.J., Griffiths M.M., Cremer M.A. The Mechanism of Autoantibody Formation to Cartilage in Rheumatoid Arthritis: Possible CrossReaction of Antibodies to Dietary Collagens with Autologous Type II Collagen. Clin Imm Immunopath, 1996; 79(2):142154(13) Terato K, Shimozuru Y, Katayama K, et al. Specificity of antibodies to type II collagen in rheumatoid arthritis. Arthritis Rheum 1990;33:1493500. Clague RB, Moore LJ. IgG and IgM antibody to native type II collagen in rheumatoid serum and synovial fluid: evidence for the presence of collagenanticollagen immune complexes in synovial fluid. Arthritis Rheum 1984;27:13707. Fujii K, Tsuji M, Kitamura A, Murota K. The diagnostic significance of antitype II collagen antibody assay in rheumatoid arthritis. Int Orthop 1992;16:2726.
ELISA kits for collagen and chondrocytes more
The term “freeze-dry” is a lay description for the process of lyophilization protein powder. It is the conversion of water from a frozen state to a gaseous state without going through a liquid state. Aliquoting with 25 mM HEPES-KOH, pH 7.2 and 1 mM DTT and 10% weight on volume glycerol is suitable for storage of the reconstituted powder. The freeze-dry process removes moisture from the cells of specimens while the specimens remain frozen.
Biotinylated Lipopolysaccharide from O111:B4, .1 mg,
Biotinylated Lipopolysaccharide from E. coli O111:B4, 0.1 mg, lyophilized