This antibody has been succesfully tested for use in following methods: Western blotting, Immunoprecipitation, Immunohistochemistry
Target of this antibody: Histone H2A
Formulation of this antibody: 100 µg (0.5 mg/ml) affinity purified rabbit anti-Histone H2A polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Alternative names of antibody target: H2AFX , H2A/X , H2A.X , H2AX , H2a/x
Recommended usage: Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml) and Immunohistochemistry (10-30 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~14 kDa histone H2A protein. It does not cross-react with other histones. HeLa cell nuclear extract (Cat.# 1641-1) can be used as a positive control.
Storage information: Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions: gel pack
Antibody host: Rabbit
This antibody reacts with: Human, mouse, rat
Clonality of antibody: Polyclonal
Isotype: Rabbit IgG
Purification method: Affinity purified
Immunogene sequence: Synthetic peptide corresponding to the N-terminus of human histone H2A
How to handle this antibody: The antibody solution should be gently mixed before use.
Background information: The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
Form of antibody: Liquid
Concentration of antibody: 0.5 mg/ml
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation anticorps