p53 (Acetyl-K370) Rabbit monoclonal antibody

• Catalog number
  BS9864M-100ul
• Price
  Please ask
• Size
  100ul

• Alternative Name
  Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53
• Immunogen
  Recombinant antibody.
• Swiss Prot
  P04637
• Reactivity
  Human,Mouse,Rat
• Applications
  WB ICC/IF
• Application_all
  WB: 1:1000-1:2000 ICC/IF: 1:50-1:200
• Product
  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  Protein A affinity purified
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  This antibody detects endogenous levels of p53 protein only when acetylated at Lys370.
• Usage
  Western blot (WB) analysis of p53 (Acetyl-K370) Rabbit mAb at 1:1000 dilution Lane 1: HepG2 treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours whole cell lysates Lane 2: HepG2 untreated whole cell lysates
• BiowMW
  ~ 53, 43 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Description
  N terminal acetylation or CH3CO as epigenetic regulation of p53 ( -K370) Rabbit monoclonal antibody by NATs.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by Bioworld. Rabbit antibodies are very stable and can be stored for several days at room temperature. Bioworld adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus
• French translation
  anticorps

• Gene target
  p53   K370   monoclonal  
• Short name
  p53 ( -K370) Rabbit monoclonal antibody
• Technique
  Antibody, Rabbit, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Rabbit, Rabbits
• Alternative name
  p53 (Acetyl-K370) production species: rabbit monoclonal (antibody to-)
• Alternative technique
  antibodies, rabbit-anti

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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