Cathepsin K, Active, mouse recombinant
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Catalog number
7597-50
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Price
Please ask
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Size
50 ug
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Synonyms
CTSK, CTSO, CTSO3
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Alternative_names
CTSK, CTSO, CTSO3
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Description
Predominant proteinase responsible for the resorption of the bone matrix.
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥90%
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Assay
SDS-PAGE
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Activity Specifications test method
The Cathepsin K is fully active as seen from its ability to cleave Cathepsin K-specific fluorescent substrate using the Cathepsin K Activity assay kit (Cat # K141-100).
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Biological activity
The specific activity is >1000 mU/mg (1U = 1 µmole/min/mg) as measured by Cathepsin K Activity Assay Kit (Catalog #K141-100)
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Results
The specific activity is >1000 mU/mg (1U = 1 µmole/min/mg) as measured by Cathepsin K Activity Assay Kit (Catalog #K141-100)
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Molecular Weight
23.4 kDa (115-329 aa)
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Storage Temp
-80°C
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Shipping
Dry Ice
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Shelf Life
6 months
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Concentration
0.5 mg/ml
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Appearance
Liquid
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Physical form description
0.5 mg/ml solution in 25 mM Na2HPO4 and 500 mM NaCl (pH 7.0) with 50% glycerol.
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Background Information
Cathepsin K, a member of the papain cysteine proteinase family is the predominant proteinase responsible for the resorption of the bone matrix. Cathepsin cleaves proteins such as collagen type I, collagen type II and osteonectin, thereby playing a role in bone remodeling and resorption in osteoporosis, osteolytic bone metastasis and rheumatoid arthritis (Bromme and Okamoto, 1995; Drake, F. et al 1996; Bossard et al, 1996).
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Gene
CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
Cathepsin K, Active, mouse recombinant
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Technique
Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Host
mouse
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Species
Mouse, Mouses
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Alternative name
Cathepsin K, functionnal, mouse Rec.
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Alternative technique
rec, murine
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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