pRb (7E6) Monoclonal Antibody

• Catalog number
  bsm-50229M
• Price
  Please ask
• Size
  0.1ml

• Long name
  pRb (7E6) Monoclonal Antibody
• Also known as
  pRb (7E6) Monoclonal Antibody
• Category
  Primary Antibodies
• Conjugation
  Unconjugated
• Target Antigen
  pRb(retinoblastoma protein)
• Specificity
  This is a highly specific antibody against pRb(retinoblastoma protein).
• Modification s
  None
• Modification site s
  Unmodified antibody
• Clonality
  Monoclonal
• Clone number
  7000000
• Concentration
  1ug per 1ul
• Source
  Recombinant human protein purified from E.coli
• Gene ID number
  5925
• Swiss Prot
  P06400
• Tested Applications
  WB
• Recommended dilutions
  WB (0.5 ug/mL) IP (2.0 ug)
• Cross reactivity
  Human
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the target antigen
  Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
• Purification method
  Purified by Protein G.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
• Synonym names
  RB; pRb; OSRC; pp110; p105-Rb; PPP1R130; Retinoblastoma-associated protein; RB1
• Properties
  If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  pRb   7E6   Monoclonal  
• Short name
  Anti-pRb(retinoblastoma protein)
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  Immunoglobulin G (IgG)1 κ
• Alternative name
  pRb (7E6) Monoclonal Antibody
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products