BAK Antibody

• Catalog number
  R30507
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  BAK
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, ICC
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the BAK antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This BAK antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q16611
• Purity
  Antigen affinity
• Description
  Bcl2 antagonist killer is a protein that in humans, encoded by the BAK gene. The protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. The gene spans 7.6 kb and contains 6 exons. By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, the gene is localized to chromosome 6. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome. BAK also interacts with the tumor suppressor P53 after exposure to cell stress.
• Immunogen
  An amino acid sequence from the middle region of human BAK (QVGRQLALIGDDINRRYDSEFQ) was used as the immunogen for this BAK antibody.
• Storage
  After reconstitution, the BAK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  BAK  
• Gene symbol
  BAK1
• Short name
  Anti-BAK
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to BAK
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:949
• Gene
  BAK1
• Long gene name
  BCL2 antagonist/killer 1
• Synonyms gene
  • CDN1
• Synonyms gene name
  • BCL2-antagonist/killer 1
• Synonyms
  • BCL2L7
  • BAK
• GenBank acession
  • U23765
• Locus
  6p21.31
• Discovery year
  1997-10-09
• Entrez gene record
  578
• Pubmed identfication
  • 7715730
  • 7715731
• RefSeq identity
  • NM_001188
• Classification
  • BCL2 family
• VEGA ID
  OTTHUMG00000014530

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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