Mouse Anti-Mouse LRP4 (Extracellular) Monoclonal IgG2A, Clone S207-27
-
Catalog numberSMC-418D-HRP
-
PricePlease ask
-
Size100 µg
-
-
CloneS207-27
-
ImmunogenFusion protein amino acids 26- 350 (extracellular N-terminus) of mouse LTP4
-
Antibody s full descriptionMouse Anti-Mouse LRP4 Monoclonal IgG2A Antibody, Clone: S207-27: HRP
-
Antibody s categoryMonoclonal Antibodies
-
Antibody s other nameCorin Antibody, KIAA0816 Antibody, LDLR Antibody, LDLR dan Antibody, Low density lipoprotein receptor related protein 4 Antibody, LRP-4 Antibody, LRP10 Antibody, MEGF7 Antibody, Multiple epidermal growth factor like domains 7 Antibody
-
Raised inMouse
-
Antibody s targetLRP4
-
Primary research fieldsNeuroscience, Cell Structure, Membrane Markers, Cell Signaling
-
BrandnameNone
-
Antibodies applicationsWB, ICC/IF
-
Antibody s reactivityHuman, Mouse, Rat
-
Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
-
PurityProtein G Purified
-
Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration1 mg/ml
-
Antibody s specificityDetects the extracellular domain of LRP4 ~215kDa. Degradation product can be seen at ~150kDa.
-
Storage recommendations-20ºC
-
Shipping recommendationsBlue Ice or 4ºC
-
Antibody certificate of analysis1 µg/ml of SMC-418 was sufficient for detection of LRP4 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
-
Antibody in cellMembrane
-
Tissue specificitySee included datasheet or contact our support service
-
Scientific contextPotential cell surface endocytic receptor, which binds and internalizes extracellular ligands for degradation by lysosomes. It is involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway.
-
Bibliography1. Bennett V., Baines A.J. (2001) Physiol. Rev. 81 (3): 1353–92.
-
Released date27-Feb-2013
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
NCBI numberNP_766256.3
-
Gene number83469
-
Protein numberQ8VI56
-
Antibody s datasheetContact our support service
-
Representative figure link
-
Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRP4 (Extracellular) Monoclonal Antibody, Clone S207-27 (SMC-418). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRP4 (Extracellular) Monoclonal Antibody (SMC-418) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) LRP4 (Extracellular) Antibody (D) Composite. Western Blot analysis of Rat brain membrane lysate showing detection of LRP4 protein using Mouse Anti-LRP4 Monoclonal Antibody, Clone S207-27 (SMC-418). Primary Antibody: Mouse Anti-LRP4 Monoclonal Antibody (SMC-418) at 1:500, 1:1000, and 1:2000. Mouse Anti-LRP4 (Extracellular) Antibody [S207-27] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-418) Mouse Anti-LRP4 Antibody [S207-27] used in Western Blot (WB) on Rat brain membrane lysate (SMC-418)
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilityIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus
-
Gene target
-
Gene symbolLRP4-AS1, SNORD115-27, IGKV1D-27, ERVK-27, ERVW-27, RNVU1-27, IGHV7-27, SNORD116-27, SNORD114-27, IGLV3-27
-
Short nameMouse Anti-Mouse LRP4 (Extracellular) Monoclonal IgG2A, Clone S207-27
-
Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
-
Hostmouse
-
IsotypeIgG2A, IgG2a
-
LabelHRP
-
SpeciesMouse, Mouses
-
Alternative nameMouse Antibody toMouse low density lipoprotein receptor-related protein 4 (Extracellular) monoclonal IgG2A, clonality S207-27
-
Alternative techniquemurine, antibodies
-
Alternative to gene targetlow density lipoprotein receptor-related protein 4, CLSS and LRP-4 and LRP10 and MEGF7 and SOST2, LRP4 and IDBG-42508 and ENSG00000134569 and 4038, scaffold protein binding, Cell surfaces, Lrp4 and IDBG-191212 and ENSMUSG00000027253 and 228357, BT.103632 and IDBG-637271 and ENSBTAG00000008429 and 504317
-
Clone nameS207-27
-
Gene info
-
Identity
-
Gene
-
Long gene nameLRP4 antisense RNA 1
-
Synonyms gene name
- LRP4 antisense RNA 1 (non-protein coding)
-
Locus
-
Discovery year2012-06-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- Antisense RNAs
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 115-27
-
Synonyms
-
Locus
-
Discovery year2011-08-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 1D-27 (pseudogene)
-
Synonyms gene name
- immunoglobulin kappa variable 1D-27
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin kappa locus at 2p11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameendogenous retrovirus group K member 27
-
Synonyms gene name
- endogenous retrovirus group K, member 27
-
GenBank acession
-
Locus
-
Discovery year2014-01-03
-
Pubmed identfication
Gene info
-
Identity
-
Gene
-
Long gene nameendogenous retrovirus group W member 27
-
Synonyms gene name
- endogenous retrovirus group W, member 27
-
GenBank acession
-
Locus
-
Discovery year2013-10-21
Gene info
-
Identity
-
Gene
-
Long gene nameRNA, variant U1 small nuclear 27
-
Locus
-
Discovery year2019-07-26
-
Classification
- Variant U1 small nuclear RNAs
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable 7-27 (pseudogene)
-
Synonyms gene name
- immunoglobulin heavy variable 7-27
- immunoglobulin heavy variable 7-27 pseudogene
-
GenBank acession
-
Locus
-
Discovery year2000-04-04
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 116-27
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2007-02-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 114-27
-
Synonyms
-
Locus
-
Discovery year2006-09-26
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin lambda variable 3-27
-
GenBank acession
-
Locus
-
Discovery year2000-05-08
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin lambda locus at 22q11.2
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
