H1, histone H1
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Catalog numberAS11 1801
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PricePlease ask
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Size50 µg
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Available ordering formatLyophilized
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Immunogennative H1 protein purified from Nicotiana tabaccum (H1A, H1B H1C,D,E,F)
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Raised inRabbit
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationAffinity purified serum
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How to reconstituteFor reconstitution add 50 µl of sterile water.
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Storage conditionstore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applicationswestern blot (WB), immunocytochemistry (ICC)
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Connected productsAS10 710 | H3 | histone H3, rabbit antibody AS11 1753 | HDT3 | histone deacetylase, rabbit antibody collection of antibodies to DNA/RNA/cell cyclePlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use1 : 5000 with standard ECL (WB), 1: 100 - 1: 500 (ICC)
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Molecular weight expected аpparent15 | 17 kDa
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Verified reactivityArabidopsis thaliana, Nicotiana tabacum, Triticum aestivum
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Possible reactivityLathyrus sativus, Phaseolus vulgaris, Pisum sativum, Solanum lycopersicum, Vicia faba
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No reactivityno confirmed exceptions from predicted reactivity known in the moment
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Supplementary informationProtocol for isolation of cytosolic and nuclear fractions can be found here.Protocol for immunostatining in whole-mount plant tissues: An efficient method for quantitative, single-cell analysis of chromatin modification and nuclear architecture in whole-mount ovules in Arabidopsis Wenjing She, Daniel Grimanelli, Célia Baroux Journal of Vizualized Experiments (JoVE), in press
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ReferencesShe and Baroux (2015). Chromatin dynamics in Pollen Mother Cells underpin a common scenario at the somatic-to-reproductive fate transition of both the male and female lineages in Arabidopsis. Front. Plant Sci. | doi: 10.3389/fpls.2015.00294. She et al. (2013). Chromatin reprogramming during the somatic to-reproductive cell fate transition in plants. Development Oct;140(19):4008-19. doi: 10.1242/dev.095034. Epub 2013 Sep 4. (Arabidopsis thaliana, immunostaining)
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Scientific contextHistone 1 (H1) is a protein located in nuclei, incorporated into chromatin.
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NotesNone
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Protein numberRefer to NCBI
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TAIR numberRefer to NCBI
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Gene target
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Gene symbolH1-0, H1-10, H1-9P
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Short nameH1, histone H1
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Alternative nameH1, histone H1
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Gene info
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Identity
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Gene
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Long gene nameH1.0 linker histone
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Synonyms gene
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Synonyms gene name
- H1 histone family, member 0
- H1 histone family member 0
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-07-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H1 histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH1.10 linker histone
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Synonyms gene
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Synonyms gene name
- H1 histone family, member X
- H1 histone family member X
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-06-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H1 histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH1.9 linker histone, pseudogene
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Synonyms gene
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Synonyms gene name
- histone linker H1 domain, spermatid-specific 1
- histone linker H1 domain, spermatid-specific 1 (pseudogene)
- H1.9 linker histone (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-12-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H1 histones
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VEGA ID