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Immunogen
A synthesized peptide derived from human Smad3
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Antibody s target
Human Smad3
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Antibody s full description
Rabbit Anti-Human Smad3 Polyclonal
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Primary research fields
Cell Signaling, Epigenetics and Nuclear Signalling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, TGF Beta, Cytoplasmic
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
hMAD 3 Antibody, JV15 2 Antibody, LDS1C Antibody, MAD (mothers against decapentaplegic Drosophila) homolog 3 Antibody, MAD homolog 3 Antibody, Mad3 Antibody, MADH3 Antibody, SMAD 3 Antibody
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Verified applications
WB, IHC
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~48kDa, endogenous levels of total Smad3
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1285 was sufficient for detection of Smad3 in 10 µg of Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Members of the Smad family of cell signaling molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. There are three distinct classes of Smads: the receptor-regulated Smads (R-Smads), the common-mediator Smad (co-Smad), and the antagonistic or inhibitory Smads. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.
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Bibliography
1. Heldin C.H. et al. (1997) Nature. 390: 465-471. 2. Attisano L. and Wrana, J.L. (1998) Curr Opin Cell Biol. 10: 188-194. 3. Massagué J. (1998) Annu Rev Biochem. 67: 753-791. 4. Whitman M. (1998) Genes Dev. 12: 2445-2462. 5. Wu G. et al. (2000) Science. 287: 92-97. 6. Abdollah S., et al. (1997) J. Biol. Chem. 272: 27678-27685. 7. Souchelnytskyi S., et al. (1997) J. Biol. Chem. 272: 28107-28115. 8. Liu X., et al. (1997) Proc. Natl. Acad. Sci. USA. 94: 10669-10674.
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Released date
6/Sep/2016
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NCBI number
NP_005893.1
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Gene number
4088
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Protein number
P84022
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Human Hek293 cell lysates showing detection of ~58kDa Smad3 protein using Rabbit Anti-Smad3 Polyclonal Antibody (SPC-1285). Lane 1: Human Hek293 treated with the immunizing peptide. Lane 2: Human Hek293. Primary Antibody: Rabbit Anti-Smad3 Polyclonal Antibody (SPC-1285) at 1:1000. Predicted/Observed Size: ~58kDa. Rabbit Anti-Smad3 Antibody used in Western blot (WB) on Hek293 cell lysates (SPC-1285)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Conjugation
not conjugated
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Gene target
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Short name
Rabbit Anti- Smad3 Polyclonal: Unconjugated
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
Unconjugated
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman SMAD family member 3 polyclonal: nonconjugated
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
SMAD family member 3, HSPC193 and HsT17436 and JV15-2 and LDS1C and LDS3 and MADH3, SMAD3 and IDBG-18366 and ENSG00000166949 and 4088, transforming growth factor beta receptor, nuclei, Smad3 and IDBG-175693 and ENSMUSG00000032402 and 17127, SMAD3 and IDBG-645466 and ENSBTAG00000012599 and 515125
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