Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric)

  • Catalog number
    K205-100
  • Price
    Please ask
  • Size
    100 assays
  • Description
    Ultrasensitive, Simple, HTP adaptable colorimetric assay kit for NOS activity measurement
  • Summary
    Ultrasensitive, Simple, HTP adaptable colorimetric assay kit for NOS activity measurement. Detection method- Colorimetry (Abs: 540 nm) Applications- Detection of NOS activity Sample Type: Purified recombinant protein, Tissue or cell extracts
  • Detection Method
    Colorimetry (Abs: 540 nm)
  • Species Reactivity
    Mammalian samples
  • Applications
    Detection of NOS activity
  • Sample Type
    • Purified recombinant protein • Tissue or cell extracts
  • Features Benefits
    Simple, HTP adaptable protocol Sensitivity as low as 5 µU of NOS activity Colorimetric detection
  • Storage Conditions
    -80°C
  • Shipping Conditions
    dry ice
  • Shelf life
    12 months
  • Background
    Nitric oxide synthases (EC 1.14.13.39) (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). nNOS accounts for the production of NO in central nervous system, where NO participates in cell communication and information storage. eNOS produces NO in blood vessels and is involved in regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as a part of body’s defense mechanism. BioVision’s Nitric Oxide Synthase Activity Assay Kit provides an accurate and convenient method to assay NOS activity in a variety of samples. In this assay, nitric oxide generated by NOS undergoes a series of reactions and reacts with Griess Reagent 1 and 2 to generate a colored product with a strong absorbance at 540 nm. The assay is simple, sensitive and high-throughput adaptable and can detect as low as 5 µU of NOS activity.
  • Data Sheet Link
  • Test
    Biovision supplies other types of Assays as 1.
  • Properties
    Colorimetric assays or detection use UV absorption or enzymatic color reaction.
  • Gene target
    Nitric   Oxide   Synthase   NOS   Activity   Kit  
  • Gene symbol
    NOS2, NOS1
  • Short name
    Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric)
  • Technique
    colorimetric, Assay, assays, colorimetry
  • Host
    Assay
  • Alternative name
    Nitric Oxide Synthase (NOS) Activity test reagent (colorimetry)
  • Alternative technique
    assays, arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
Gene info
Product images
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