Recombinant Human CKMT1A Protein (His tag)

• Catalog number
  PKSH030325-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  CKMT;CKMT1;CKMT1A
• Activity
  NA
• Sequence
  Ala40-His417
• Fusion tag
  N-His
• Accession
  P12532-1
• Expressed Host
  Baculovirus-Insect Cells
• Shipping
  Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
• Purity
  ＞95 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  45.3 kDa
• AP Mol Mass
  43 kDa
• Formulation
  Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% gly
• Background
  CKMT1A belongs to the ATP:guanido phosphotransferase family. It contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. CKMT1A gene is one of two genes which encode the ubiquitous mitochondrial creatine kinase (CKMT1). CKMT1 is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK (CKMT2) and ubiquitous MtCK, encoded by separate genes. CKMT1 occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric CKMT1.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CKMT1A   Protein   His   tag  
• Gene symbol
  CKMT1A
• Short name
  Recombinant CKMT1A Protein (His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens CKMT1A Protein (histidine detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:31736
• Gene
  CKMT1A
• Long gene name
  creatine kinase, mitochondrial 1A
• Synonyms gene
  • CKMT1
• Synonyms gene name
  • creatine kinase, mitochondrial 1 (ubiquitous)
• GenBank acession
  • BC001926
  • BC006467
• Locus
  15q15.3
• Discovery year
  2005-04-15
• Entrez gene record
  548596
• VEGA ID
  OTTHUMG00000059898

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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