Chymotrypsinogen B1, Human Recombinant

• Catalog number
  P1226-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  CTRB1, CTRB
• Alternative_names
  CTRB1, CTRB
• Description
  Precursor of the pancreatic proteolytic enzymes
• Recombinant
  Yes
• Source
  E.coli
• Purity by SDS PAGE
  > 95%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by its ability to hydrolyze 1.0 umole of N-alpha-acetyl-L-tyrosine ethyl ester (ATEE) per min at pH 7.0 at 25°C
• Biological activity
  Specifiv activity 1100 units/mg
• Results
  1100 units/mg
• Unit Definition
  One unit is defined as the amount of enzyme that will hydrolyze 1.0 umole of N-alpha-acetyl-L-tyrosine ethyl ester (ATEE) per min at pH 7.0 at 25°C.
• Molecular Weight
  27 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized without any additives.
• Reconstitution Instructions
  Reconstitute the lyophilized Human CTRB1 in 1 ml 50 mM HAc which can then be further diluted to other aqueous solutions.
• Background Information
  Chymotrypsinogen-B1 (CTRB1) belongs to the serine protease family of enzymes and forms a main precursor of the pancreatic proteolytic enzymes. CTRB1 is located next to a related chymotrypsinogen gene. CTRB1 is a protein coding gene which encodes different isoforms which may undergo similar processing to generate the mature protein.
• Amino acid sequence
  CG VPAIHPVLSG LSRIVNGEDA VPGSWPWQVS LQDKTGFHFC GGSLISEDWV VTAAHCGVRT SDVVVAGEFD QGSDEENIQV LKIAKVFKNP KFSILTVNND ITLLKLATPA RFSQTVSAVC LPSADDDFPAGTLCATTGWG KTKYNANKTP DKLQQAALPL LSNAECKKSW GRRITDVMIC AGASGVSSCM GDSGGPLVCQ KDGAWTLVGI VSWGSDTCST SSPGVYARVTKLIPWVQKIL AAN
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Chymotrypsinogen  
• Gene symbol
  CTRB1
• Short name
  Chymotrypsinogen B1, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Chymotrypsinogen B1, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:2521
• Gene
  CTRB1
• Long gene name
  chymotrypsinogen B1
• Synonyms gene
  • CTRB
• Locus
  16q23.1
• Discovery year
  1986-01-01
• Entrez gene record
  1504
• Pubmed identfication
  • 2917002
  • 8186414
• RefSeq identity
  • NM_001906
• VEGA ID
  OTTHUMG00000159272

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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