Human MAP2K1(Mitogen Activated Protein Kinase Kinase 1) EIA Kit

• Catalog number
  E-EL-H0505-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Human
• Target Synonym
  MAPKK1, MEK1, MKK1, PRKMK1
• Test principle
  This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human MAP2K1. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MAP2K1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAP2K1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MAP2K1. You can calculate the concentration of Human MAP2K1 in the samples by comparing the OD of the samples to the standard curve.
• Sensitivity
  0.1ng/mL
• Detection Range
  0.16~10ng/mL
• Target Name
  MAP2K1
• UNIProt ID
  Q02750
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Human MAP2K1(Mitogen Activated Protein Kinase Kinase 1) EIA Kit
• Type
  Sandwich
• Technical data
• Standard Curve RUL
• Description
  This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
• Properties
  MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

• Gene target
  MAP2K1   Mitogen   Activated   Protein   Kinase   Kinase   Kit  
• Gene symbol
  MAP2K1
• Short name
  MAP2K1(Mitogen Activated Protein Kinase Kinase 1) EIA Kit
• Technique
  EIA, eia
• Species
  Human, Humans
• Alternative name
  H. sapiens mitogen-activated protein kinase kinase 1(Mitogen Activated Protein phosphorylation catalyst phosphorylation catalyst 1) EIA reagent
• Alternative technique
  kits, elisas
• Alternative to gene target
  mitogen-activated protein kinase kinase 1, CFC3 and MAPKK1 and MEK1 and MKK1 and PRKMK1, MAP2K1 and IDBG-18019 and ENSG00000169032 and 5604, transferase activity, nuclei, Map2k1 and IDBG-176099 and ENSMUSG00000004936 and 26395, MAP2K1 and IDBG-645446 and ENSBTAG00000033983 and 533199

Gene info

• Identity
  HGNC:6840
• Gene
  MAP2K1
• Long gene name
  mitogen-activated protein kinase kinase 1
• Synonyms gene
  • PRKMK1
• Synonyms
  • MEK1
  • MAPKK1
• GenBank acession
  • L11284
• Locus
  15q22.31
• Discovery year
  1993-11-05
• Entrez gene record
  5604
• Pubmed identfication
  • 9465908
  • 8388392
• Classification
  • Mitogen-activated protein kinase kinases
• VEGA ID
  OTTHUMG00000133196
• Locus Specific Databases
  • The Globin Gene Server
  • LRG_725

MeSH Data

• Name
  Immunoenzyme Techniques
• Concept
  Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
• Tree numbers
  • E05.478.566.350
  • E05.478.583.400
  • E05.601.470.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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