UltraPolymer Goat Anti-Rabbit IgG (H&L) HRP
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Catalog number
A1274-5
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Price
Please ask
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Size
5 ml
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Antibody Target
Rabbit IgG
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Target Alternative Name
Anti-rabbit IgG, HRP-Linked Antibody, HRP linked secondary antibody
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Antibody Type
Secondary antibody
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Species Reactivities
Rabbit
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Immunogen Sequence
Rabbit IgG (H&L)
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
In PBS with 1% BSA and 0.1% proclin 150
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Purification
Affinity Purified
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Application
IHC
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Positive Control
IHC: Breast cancer tissue
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Application And Usages
IHC: 120-150 µl
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Description
UltraPolymer Goat anti-Rabbit IgG (H&L) conjugated to HRP, affinity purified, min cross-reactivity with human, mouse or rat serum
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Storage Condition
4 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Not Verified
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Immunocytochemistry Verified
Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Latin name
Capra aegagrus hircus, Oryctolagus cuniculus
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
HRP Conjugated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Gene target
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Short name
UltraPolymer Goat Anti-Rabbit IgG (H L)
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Technique
Rabbit, anti-, anti, Goat, IgG, antibody to, antibodies, goats, IgGs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Isotype
Goat IgG, Heavy & Light chains, IgG
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Label
HRP
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Species
Goats
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Alternative name
UltraPolymer caprine Antibody tohost: rabbit Immunoglobulin G (heavy + light chain) horseradish peroxidase
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Alternative technique
rabbit-anti, antibodies, caprine, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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